Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC. 2012. Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Online]. PLOS One. Available from: https://doi.org/10.1371/journal.pone.0041888

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC. Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment [Internet]. PLOS One; 2012. Available from: https://doi.org/10.1371/journal.pone.0041888

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC (2012). Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Data Collection]. PLOS One. https://doi.org/10.1371/journal.pone.0041888

Description

Keywords

Methods

Data capture method Experiment: Laboratory
Date (Date published in a 3rd party system) 7 August 2012
Language(s) of written materials English

People & Groups

Data Creators Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC
LSHTM Faculty/Department Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Participating Institutions London School of Hygiene & Tropical Medicine, London, United Kingdom, Liverpool School of Tropical Medicine, Liverpool, United Kingdom, Laboratorio de Proteinomica Estructua

Projects

Funders
ProjectFunderGrant NumberFunder URI
UNSPECIFIEDBiotechnology & Biological Sciences Research CouncilBB/F012675/1UNSPECIFIED

Additional Information

Date Deposited 12 Jan 2016 10:23
Last Modified 27 Apr 2022 18:20
Publisher PLOS One

Share

Downloads

Data / Code

Table_S1 Download

Filename: Table_S1.docx

Description: Minimum information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines. Guidelines published by Bustin et al 2009 were referred to in order to ensure accuracy and reliability of quantitative PCR data

Licence:

Content type: Textual content

File size: 21kB

Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document

Download

[img]

Table_S2 Download

Filename: Table_S2.docx

Description: Raw individual relative gene expression data generated by quantitative PCR. Raw qPCR data generated from relative expression analysis to show fold changes in expression of venom genes of interest, including snake venom metalloproteinase (SVMP)

Licence:

Content type: Dataset

File size: 19kB

Mime-Type: application/vnd.openxmlformats-officedocument.wordprocessingml.document

Download

[img]

Data Visualisation

Figure_S1 Download

Filename: Figure_S1.tif

Description: Optimisation of venom quantitative PCR. Representative standard curves for snake venom metalloproteinase (SVMP) and C-type lectin (CTL) (1Ai and Aii) show high efficiency amplification of 94.0 and 96.6% respectively.

Licence:

Content type: Still Image

File size: 646kB

Mime-Type: image/tiff

Download

[img]

Figure_S2 Download

Filename: Figure_S2.tif

Description: Raw data following gene expression analysis by quantitative PCR. Gene expression analysis conducted using the BioRad CFX manager software

Licence:

Content type: Still Image

File size: 1MB

Mime-Type: image/tiff

Download

[img]

External resources

Data record - Figshare (Data)

Publication - PLOS One (Online Data Resource)

Downloads

View details

Metrics & Citations

Google Scholar

Google Scholar
Report this Record Edit item (admin only)