Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC (2012). Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Dataset]. PLOS One. https://doi.org/10.1371/journal.pone.0041888
Copy

Data to support a publication on the dynamics of gene expression in synthesised venom proteins following venom depletion. Outputs include a gene expression analysis generated by quantitative PCR and minimum information on on Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines.

Keywords

RNA, Snake venom, Venom replenishment

Item Type Dataset
Capture method Experiment: Laboratory
Date 7 August 2012
Language(s) of written materials English
Creator(s) Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC
LSHTM Faculty/Department Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology (-2019)
Participating Institutions London School of Hygiene & Tropical Medicine, London, United Kingdom, Liverpool School of Tropical Medicine, Liverpool, United Kingdom, Laboratorio de Proteinomica Estructua
Funders
Project
Funder
Grant Number
Funder URI
UNSPECIFIED
Biotechnology & Biological Sciences Research Council
BB/F012675/1
UNSPECIFIED
Date Deposited 12 Jan 2016 10:23
Last Modified 27 Apr 2022 18:20
Publisher PLOS One

Explore Further

Figure_S1.tif
subject
Graphical representation of data
Creative Commons: Attribution
Available under Creative Commons: Attribution 3.0
info
Optimisation of venom quantitative PCR. Representative standard curves for snake venom metalloproteinase (SVMP) and C-type lectin (CTL) (1Ai and Aii) show high efficiency amplification of 94.0 and 96.6% respectively.
description
image/tiff
folder_info
646kB
Download
Figure_S2.tif
subject
Graphical representation of data
Creative Commons: Attribution
Available under Creative Commons: Attribution 3.0
info
Raw data following gene expression analysis by quantitative PCR. Gene expression analysis conducted using the BioRad CFX manager software
description
image/tiff
folder_info
1MB
Download
Table_S1.docx
subject
Data
Creative Commons: Attribution
Available under Creative Commons: Attribution 3.0
info
Minimum information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines. Guidelines published by Bustin et al 2009 were referred to in order to ensure accuracy and reliability of quantitative PCR data
description
application/vnd.openxmlformats-officedocument.wordprocessingml.document
folder_info
21kB
Download
Table_S2.docx
subject
Data
Creative Commons: Attribution
Available under Creative Commons: Attribution 3.0
info
Raw individual relative gene expression data generated by quantitative PCR. Raw qPCR data generated from relative expression analysis to show fold changes in expression of venom genes of interest, including snake venom metalloproteinase (SVMP)
description
application/vnd.openxmlformats-officedocument.wordprocessingml.document
folder_info
19kB
Download

Atom BibTeX OpenURL ContextObject in Span Multiline CSV OpenURL ContextObject Dublin Core (with Type as Type) MPEG-21 DIDL Data Cite XML EndNote HTML Citation JSON METS MODS RDF+N3 RDF+N-Triples RDF+XML Reference Manager Refer Simple Metadata ASCII Citation EP3 XML
Export

Downloads