Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC. 2012. Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Online]. Figshare. Available from: https://doi.org/10.1371/journal.pone.0041888.

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC. Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Internet] LSHTM Data Compass. Figshare; 2012. Available from: https://doi.org/10.1371/journal.pone.0041888.

Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC (2012). Unusual stability of messenger RNA in snake venom reveals gene expression dynamics of venom replenishment. [Data Collection]. Figshare. https://doi.org/10.1371/journal.pone.0041888.

Description

Data capture method Experiment: Laboratory
Date (Published in a 3rd party system) 7 August 2012
Language(s) of written materials English
Data Creators Currier, R, Calvete, JJ, Sanz, L, Harrison, RA, Rowley, PD and Wagstaff, SC
LSHTM Faculty/Department Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Participating Institutions London School of Hygiene & Tropical Medicine, Liverpool School of Tropical Medicine, Laboratorio de Proteinomica Estructua
Funders
ProjectFunderGrant NumberFunder URI
UNSPECIFIEDUNSPECIFIEDBB/F012675/1UNSPECIFIED
Depositor Gareth Knight
Date Deposited 12 Jan 2016 10:23
Last Modified 05 Aug 2016 14:52
Publisher Figshare

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Data

Filename: Table_S1.docx

Description: Minimum information for Publication of Quantitative Real-time PCR Experiments (MIQE) guidelines. Guidelines published by Bustin et al 2009 were referred to in order to ensure accuracy and reliability of quantitative PCR data

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File size: 21kB

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Filename: Table_S2.docx

Description: Raw individual relative gene expression data generated by quantitative PCR. Raw qPCR data generated from relative expression analysis to show fold changes in expression of venom genes of interest, including snake venom metalloproteinase (SVMP)

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Content type: Dataset

File size: 19kB

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Data Visualisation

Filename: Figure_S1.tif

Description: Optimisation of venom quantitative PCR. Representative standard curves for snake venom metalloproteinase (SVMP) and C-type lectin (CTL) (1Ai and Aii) show high efficiency amplification of 94.0 and 96.6% respectively.

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File size: 646kB

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Filename: Figure_S2.tif

Description: Raw data following gene expression analysis by quantitative PCR. Gene expression analysis conducted using the BioRad CFX manager software

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File size: 1MB

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