Identification and characterisation of bacterial TIR domains, with particular focus on yersinia pestis: Study Data

Spear, AM (2011). Identification and characterisation of bacterial TIR domains, with particular focus on yersinia pestis: Study Data. [Dataset]. London School of Hygiene & Tropical Medicine, London, United Kingdom. 10.17037/DATA.00000080.
Copy

The TolllIL-l Receptor (TIR) domain is an essential signalling module in eukaryotic innate immune signalling pathways. Homotypic interaction between TIR domains allows the formation of a signalling platform in which molecules are able to interact and activate each other to initiate an immune signalling cascade. Proteins containing TIR domains have also been discovered in bacteria. Studies have subsequently shown that these proteins are able to modulate mammalian immune signalling pathways dependent on TIR interactions and that this forms an evasion strategy for bacterial pathogens. In this study a bioinformatic search for proteins containing TIR domains was carried out across unicellular organisms, including bacteria. TIR domain proteins (Tdps) from highly pathogenic bacteria were down-selected for investigation. After an initial screen of their activity, a Tdp from Yersinia pestis, the causative agent of plague, was down-selected for further investigation. The bioinformatic analysis found a high representation of Tdps in bacteria generally classified as non-pathogens, and that TIR domains are promiscuous in their co-occurrence with other domains. This analysis also showed that they are not necessarily conserved between strains and species. These findings question the universal role of Tdps in the pathogenic evasion of a host immune response and suggest they may have other functions. Initial screening of down-selected Tdps showed that they were able to modulate immune signalling pathways in vitro, but studies with a Tdp-deficient mutant of Y. pestis did not demonstrate a role for this protein in the virulence of Y. pestis in a mouse model. However, this Tdp-deficient mutant did display two characteristics in vitro: an increased autoaggregation phenotype when compared to wild-type Y. pestis and an inability to survive as well as wild-type bacteria in conditions of high salinity. These fmdings indicate that TIR domain proteins may have other roles in bacterial physiology unrelated to immune evasion.

Keywords

TolllIL-l Receptor, Yersinia pestis, Mammalian immune signalling pathways

AppendixB.xlsx
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
Full list of PSI-BLAST protein results, including archaeal, bacterial and summary tables
grid_on
application/vnd.openxmlformats-officedocument.spreadsheetml.sheet
folder_info
132kB

Download
AppendixB-ArchaealResults.csv
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
Archaeal results dataset. Exported from Appendix B Excel spreadsheet
description
text/plain
folder_info
1kB

Download
AppendixB-BacterialResult.csv
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
Bacterial results dataset. Exported from Appendix B Excel spreadsheet
description
text/plain
folder_info
239kB

Download
AppendixB-SuppTable3-Summary.csv
subject
Data
Creative Commons: Attribution-Noncommercial-Share Alike
Available under Creative Commons: Attribution-Noncommercial-Share Alike 3.0
info
Supplementary table 3 - see thesis for further details
description
text/plain
folder_info
7kB

Download
BaTdp-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
BaTdp sequence files
folder_zip
application/x-zip
folder_info
339kB

Download
BmTdp2-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
BmTdp2 sequence files
folder_zip
application/x-zip
folder_info
235kB

Download
BmTdp-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
BmTdp sequence files
folder_zip
application/x-zip
folder_info
298kB

Download
BpTdp-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
BpTdp sequence files
folder_zip
application/x-zip
folder_info
406kB

Download
BthTdp-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
BthTdp sequence files
folder_zip
application/x-zip
folder_info
387kB

Download
YpTdp-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
YpTdp sequence files
folder_zip
application/x-zip
folder_info
314kB

Download
YpTIR-sequence.zip
subject
Data
Creative Commons: Attribution-Noncommercial-No Derivative Works
Available under Creative Commons: Attribution-Noncommercial-No Derivative Works 3.0
info
YpTIR sequence files
folder_zip
application/x-zip
folder_info
303kB

Download

Atom BibTeX OpenURL ContextObject in Span Multiline CSV OpenURL ContextObject Dublin Core (with Type as Type) MPEG-21 DIDL Data Cite XML EndNote HTML Citation JSON METS MODS RDF+N3 RDF+N-Triples RDF+XML Reference Manager Refer Simple Metadata ASCII Citation EP3 XML
Export

Downloads