Ijaz, UZ, Gundogdu, O, Keating, C, Eekert, Mv, Gibson, W, Parkhill, J, Abilahi, F, Liseki, B, Nguyen, V, Sudgen, S, Quince, C, Ensink, JHJ, Torondel, B and Walker, AW. 2022. Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate. [Online]. Frontiers. Available from: https://doi.org/10.3389/fmicb.2022.960747.s008
Ijaz, UZ, Gundogdu, O, Keating, C, Eekert, Mv, Gibson, W, Parkhill, J, Abilahi, F, Liseki, B, Nguyen, V, Sudgen, S, Quince, C, Ensink, JHJ, Torondel, B and Walker, AW. Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate [Internet]. Frontiers; 2022. Available from: https://doi.org/10.3389/fmicb.2022.960747.s008
Ijaz, UZ, Gundogdu, O, Keating, C, Eekert, Mv, Gibson, W, Parkhill, J, Abilahi, F, Liseki, B, Nguyen, V, Sudgen, S, Quince, C, Ensink, JHJ, Torondel, B and Walker, AW (2022). Analysis of pit latrine microbiota reveals depth-related variation in composition, and key parameters and taxa associated with latrine fill-up rate. [Data Collection]. Frontiers. https://doi.org/10.3389/fmicb.2022.960747.s008
Description
Pit latrines are used by billions of people globally, often in developing countries where they provide a low-tech and low-cost sanitation method. However, health and social problems can arise from a lack of emptying or maintenance of these facilities. A better understanding of the biological and environmental parameters within pit latrines could inform attempts to enhance material decomposition rates, and therefore slow fill-up rate. In this study, we have performed a spatial analysis of 35 Tanzanian pit latrines to identify bacteria and environmental factors that are associated with faster or slower pit latrine fill-up rates. Using ordination of microbial community data, we observed a linear gradient in terms of beta diversity with increasing pit latrine sample depth, corresponding to a shift in microbial community structure from gut-associated families in the top layer to environmental- and wastewater-associated taxa at greater depths. We also investigated the bacteria and environmental parameters associated with fill-up rates, and identified pH, volatile solids, and volatile fatty acids as features strongly positively correlated with pit latrine fill-up rates, whereas phosphate was strongly negatively correlated with fill-up rate. A number of pit latrine microbiota taxa were also correlated with fill-up rates. Using a multivariate regression, we identified the Lactobacillaceae and Incertae_Sedis_XIII taxa as particularly strongly positively and negatively correlated with fill-up rate, respectively. This study therefore increases knowledge of the microbiota within pit latrines, and identifies potentially important bacteria and environmental variables associated with fill-up rates. These new insights may be useful for future studies investigating the decomposition process within pit latrines.
Keywords
Data capture method | Unknown |
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Date (Date published in a 3rd party system) | 23 September 2022 |
Language(s) of written materials | English |
Data Creators | Ijaz, UZ, Gundogdu, O, Keating, C, Eekert, Mv, Gibson, W, Parkhill, J, Abilahi, F, Liseki, B, Nguyen, V, Sudgen, S, Quince, C, Ensink, JHJ, Torondel, B and Walker, AW |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Department of Infection Biology Faculty of Infectious and Tropical Diseases > Dept of Disease Control |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 21 Oct 2022 15:42 |
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Last Modified | 21 Oct 2022 15:42 |
Publisher | Frontiers |