Sequence of the UbeK trypanosomal degron

Borg, Y; Alsford, SORCID logo; Zaikin, A and Nesbeth, D (2021). Sequence of the UbeK trypanosomal degron. [Dataset]. Figshare. https://doi.org/10.6084/m9.figshare.14845035.v1
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Sequence of the UbeK degron signal used to decrease the half life of GFP expressed in Trypanosoma brucei brucei (T. b. brucei). The UbeK degron consists of 76 residues of native T. b. brucei ubiquitin protein, at the N-terminus and ending in a leucine residue to signal proteasomal degradation, followed by a short ‘eK’ region which featuring an additional proteasomal degradation signal of two lysine residues flanking an arginine.

Keywords

Trypanosoma brucei brucei; Degron signal; Synthetic biology

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