Fennell, TG, Blackwell, GA, Thomson, NR and Dorman, MJ. 2021. Supporting data for "chiA and gbpA genes are not uniformly distributed amongst diverse Vibrio cholerae". [Online]. Figshare. Available from: https://doi.org/10.6084/m9.figshare.13169189
Fennell, TG, Blackwell, GA, Thomson, NR and Dorman, MJ. Supporting data for "chiA and gbpA genes are not uniformly distributed amongst diverse Vibrio cholerae" [Internet]. Figshare; 2021. Available from: https://doi.org/10.6084/m9.figshare.13169189
Fennell, TG, Blackwell, GA, Thomson, NR and Dorman, MJ (2021). Supporting data for "chiA and gbpA genes are not uniformly distributed amongst diverse Vibrio cholerae". [Data Collection]. Figshare. https://doi.org/10.6084/m9.figshare.13169189
Description
Members of the bacterial genus Vibrio utilize chitin both as a metabolic substrate and a signal to activate natural competence. Vibrio cholerae is a bacterial enteric pathogen, sub-lineages of which can cause pandemic cholera. However, the chitin metabolic pathway in V. cholerae has been dissected using only a limited number of laboratory strains of this species. Here, we survey the complement of key chitin metabolism genes amongst 195 diverse V. cholerae. We show that the gene encoding GbpA, known to be an important colonization and virulence factor in pandemic isolates, is not ubiquitous amongst V. cholerae. We also identify a putatively novel chitinase, and present experimental evidence in support of its functionality. Our data indicate that the chitin metabolic pathway within V. cholerae is more complex than previously thought, and emphasize the importance of considering genes and functions in the context of a species in its entirety, rather than simply relying on traditional reference strains.
Keywords
Data capture method | Experiment: Laboratory |
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Date (Date published in a 3rd party system) | 7 May 2021 |
Language(s) of written materials | English |
Data Creators | Fennell, TG, Blackwell, GA, Thomson, NR and Dorman, MJ |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 01 Sep 2021 08:59 |
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Last Modified | 30 Sep 2021 14:16 |
Publisher | Figshare |