Nliwasa, M, MacPherson, P, Chisala, P, Kamdolozi, M, Khundi, MJ, Kaswaswa, K, Mwapasa, M, Msefula, C, Sohn, H, Flach, C and Corbett, E. 2016. LAMP minimal dataset. [Online]. PLOS ONE. Available from: https://doi.org/10.1371/journal.pone.0155101.s001
Nliwasa, M, MacPherson, P, Chisala, P, Kamdolozi, M, Khundi, MJ, Kaswaswa, K, Mwapasa, M, Msefula, C, Sohn, H, Flach, C and Corbett, E. LAMP minimal dataset [Internet]. PLOS ONE; 2016. Available from: https://doi.org/10.1371/journal.pone.0155101.s001
Nliwasa, M, MacPherson, P, Chisala, P, Kamdolozi, M, Khundi, MJ, Kaswaswa, K, Mwapasa, M, Msefula, C, Sohn, H, Flach, C and Corbett, E (2016). LAMP minimal dataset. [Data Collection]. PLOS ONE. https://doi.org/10.1371/journal.pone.0155101.s001
Description
Current tuberculosis diagnostics lack sensitivity, and are expensive. Highly accurate, rapid and cheaper diagnostic tests are required for point of care use in low resource settings with high HIV prevalence. To investigate the sensitivity and specificity, and cost of loop-mediated isothermal amplification (LAMP) assay for tuberculosis diagnosis in adults with chronic cough compared to Xpert® MTB/RIF, fluorescence smear microscopy. Between October 2013 and March 2014, consecutive adults at a primary care clinic were screened for cough, offered HIV testing and assessed for tuberculosis using LAMP, Xpert® MTB/RIF and fluorescence smear microscopy. Sensitivity and specificity (with culture as reference standard), and costs were estimated. Of 273 adults recruited, 44.3% (121/273) were HIV-positive and 19.4% (53/273) had bacteriogically confirmed tuberculosis. The sensitivity of LAMP compared to culture was 65.0% (95% CI: 48.3% to 79.4%) with 100% (95% CI: 98.0% to 100%) specificity. The sensitivity of Xpert® MTB/RIF (77.5%, 95% CI: 61.5% to 89.2%) was similar to that of LAMP, p = 0.132. The sensitivity of concentrated fluorescence smear microscopy with routine double reading (87.5%, 95% CI: 73.2% to 95.8%) was higher than that of LAMP, p = 0.020. All three tests had high specificity. The lowest cost per test of LAMP was at batch size of 14 samples (US$ 9.98); this was lower than Xpert® MTB/RIF (US$ 13.38) but higher than fluorescence smear microscopy (US$ 0.65). The sensitivity of LAMP was similar to Xpert® MTB/RIF but lower than fluorescence smear microscopy; all three tests had high specificity. These findings support the Malawi policy that recommends a combination of fluorescence smear microscopy and Xpert® MTB/RIF prioritised for people living with HIV, already found to be smear-negative, or being considered for retreatment of tuberculosis.
Description of data capture | Between October 2013 and March 2014, consecutive adults at a primary care clinic were screened for cough, offered HIV testing and assessed for tuberculosis using LAMP, Xpert MTB/RIF and fluorescence smear microscopy. Sensitivity and specificity (with culture as reference standard), and costs were estimated. | ||||||||
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Data capture method | Experiment: Field Intervention | ||||||||
Data Collection Period |
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Date (Date published in a 3rd party system) | 12 May 2016 | ||||||||
Geographical area covered (offline during plugin upgrade) |
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Language(s) of written materials | English |
Data Creators | Nliwasa, M, MacPherson, P, Chisala, P, Kamdolozi, M, Khundi, MJ, Kaswaswa, K, Mwapasa, M, Msefula, C, Sohn, H, Flach, C and Corbett, E |
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LSHTM Faculty/Department | Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology Faculty of Infectious and Tropical Diseases > Dept of Clinical Research |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom, Liverpool School of Tropical Medicine, Liverpool, United Kingdom, College of Medicine, Blantyre, Malawi, McGill University, Montreal, Quebec, Canada |
Funders |
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Date Deposited | 18 May 2016 11:14 |
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Last Modified | 20 Jul 2023 10:35 |
Publisher | PLOS ONE |