Generation of Antigenic Diversity in Plasmodium falciparum by Structured Rearrangement of Var Genes During Mitosis

Claessens, A, Hamilton, WL, Kekre, M, Otto, TD, Faizullabhoy, A, Rayner, JC and Kwiatkowski, D. 2014. Generation of Antigenic Diversity in Plasmodium falciparum by Structured Rearrangement of Var Genes During Mitosis. [Online]. PLOS Genetics. Available from: https://doi.org/10.1371/journal.pgen.1004812.

Claessens, A, Hamilton, WL, Kekre, M, Otto, TD, Faizullabhoy, A, Rayner, JC and Kwiatkowski, D. Generation of Antigenic Diversity in Plasmodium falciparum by Structured Rearrangement of Var Genes During Mitosis. [Internet] LSHTM Data Compass. PLOS Genetics; 2014. Available from: https://doi.org/10.1371/journal.pgen.1004812.

Claessens, A, Hamilton, WL, Kekre, M, Otto, TD, Faizullabhoy, A, Rayner, JC and Kwiatkowski, D (2014). Generation of Antigenic Diversity in Plasmodium falciparum by Structured Rearrangement of Var Genes During Mitosis. [Data Collection]. PLOS Genetics. https://doi.org/10.1371/journal.pgen.1004812.

Description

Data capture method Experiment: Laboratory
Date (Published in a 3rd party system) 18 December 2014
Language(s) of written materials English
Data Creators Claessens, A, Hamilton, WL, Kekre, M, Otto, TD, Faizullabhoy, A, Rayner, JC and Kwiatkowski, D
LSHTM Faculty/Department Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Research Centre Malaria Centre
Participating Institutions Wellcome Trust Sanger Institute, University of Oxford, London School of Hygiene & Tropical Medicine
Depositor Gareth Knight
Date Deposited 20 Oct 2015 09:51
Last Modified 05 Aug 2016 14:12
Publisher PLOS Genetics

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Data

Filename: S1_Table.xlsx

Description: Genome coverage for each subclone

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File size: 70kB

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Filename: S2_Table.xlsx

Description: SNPs identified in clone trees

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File size: 69kB

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Filename: S3_Table.xlsx

Description: Structural variants identified in the 3D7 clone tree, represented in Fig. 1

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File size: 141kB

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Filename: S4_Table.xlsx

Description: All var gene recombinations

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File size: 96kB

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Filename: S5_Table.xlsx

Description: Mitotic point mutation rates and var gene recombination rates measured from clone trees

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File size: 55kB

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Filename: S6_Table.xlsx

Description: Primers used for validating point mutations and var gene recombination by PCR amplification and capillary sequencing

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File size: 11kB

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Filename: S7_Table.xlsx

Description: Crosses progeny culture time and var gene recombinations

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File size: 38kB

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Data Visualisation

Filename: S1_Fig.pdf

Description: Generating clone trees

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Content type: Textual content

File size: 782kB

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Filename: S2_Fig.pdf

Description: Chimeric var gene created by recombination

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Content type: Still Image

File size: 336kB

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Filename: S3_Fig.pdf

Description: Example of 7 recombination events within var gene exon2 in 3D7_1b

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Filename: S4_Fig.pdf

Description: Triple recombination event within less than 50 bp

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File size: 323kB

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Filename: S5_Fig.pdf

Description: Duplications in internal var gene clusters can also generate chimeras

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File size: 703kB

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Filename: S6_Fig.pdf

Description: Another example of internal var gene clusters generating chimeras

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File size: 602kB

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Filename: S7_Fig.pdf

Description: Evolution of a var gene

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File size: 583kB

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Filename: S10_Fig.pdf

Description: Defining identity blocks. Var B/C genes recombine (A) ClustalW alignment of Dd2var34 and Dd2var45 at a crossover

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File size: 186kB

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Filename: S13_Fig.eps

Description: Distribution of var exon 1 recombination within each domain class

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File size: 469kB

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Filename: S14_Fig.eps

Description: No recombination hotspot within DBL domains

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Content type: Still Image

File size: 181kB

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Filename: S15_Fig.pdf

Description: No conserved motifs within Identity Blocks.

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Content type: Still Image

File size: 173kB

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Filename: S8_Fig.png

Description: All var exon 1 translocations identified in this study

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File size: 4MB

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Filename: S9_Fig.png

Description: Group B/C var genes do not necessarily recombine with var genes of highest similarity

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File size: 2MB

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Filename: S11_Fig.png

Description: Recombination in var genes occurs where sequences are similar

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File size: 1MB

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Filename: S12_Fig.png

Description: Var gene exon 1 recombine in short regions of higher homology

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File size: 4MB

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