Transcript abundance comparison between BALB/c ears inoculated with Leishmania mexicana and Leishmania mexicana plus promastigote secretory gel (PSG)

Giraud, E, Rogers, M, Bates, P and Dillon, R. 2014. Transcript abundance comparison between BALB/c ears inoculated with Leishmania mexicana and Leishmania mexicana plus promastigote secretory gel (PSG). [Online]. ArrayExpress. Available from: http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52101/

Giraud, E, Rogers, M, Bates, P and Dillon, R. Transcript abundance comparison between BALB/c ears inoculated with Leishmania mexicana and Leishmania mexicana plus promastigote secretory gel (PSG) [Internet]. ArrayExpress; 2014. Available from: http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52101/

Giraud, E, Rogers, M, Bates, P and Dillon, R (2014). Transcript abundance comparison between BALB/c ears inoculated with Leishmania mexicana and Leishmania mexicana plus promastigote secretory gel (PSG). [Data Collection]. ArrayExpress. http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-52101/

Description

To determine the modulation of gene expression of Leishmania mexicana(M379)-inoculated BALB/c ears in the presence of promastigote secretory gel (PSG) A genome-wide transcriptional analysis was performed by comparing the gene expression profiles of Leishmania mexicana- inoculated BALB/c ears and Leishmania mexicana plus PSG BALB/c ears. Leishmania mexicana amastigotes were purified from mouse cutaneous lesions and transformed in vitro in metacycic promastigotes (MT). After 6, 24 and 48 hours, ears were collected and processed for RNA extraction. Three Biological replicates per condition were run.

Methods

Data capture method	Experiment
Date (Date published in a 3rd party system)	3 June 2014
Language(s) of written materials	English

People & Groups

Data Creators	Giraud, E, Rogers, M, Bates, P and Dillon, R
LSHTM Faculty/Department	Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Participating Institutions	London School of Hygiene & Tropical Medicine