Data for: Performance of alternative diagnostic tests; Loop Isothermal Amplification Assay (LAMP), CFW+KOH, and a Smartphone based microscope-Dipple in the diagnosis of Fungal Keratitis among Patients attending MURHEC- Southwestern Uganda
Arunga, S
; Orishaba, F; Ebong, A; Kassaza, K and Karuhanga, N
(2026).
Data for: Performance of alternative diagnostic tests; Loop Isothermal Amplification Assay (LAMP), CFW+KOH, and a Smartphone based microscope-Dipple in the diagnosis of Fungal Keratitis among Patients attending MURHEC- Southwestern Uganda.
[Dataset].
London School of Hygiene & Tropical Medicine, London, United Kingdom.
10.17037/DATA.00005249.
; Orishaba, F; Ebong, A; Kassaza, K and Karuhanga, N
(2026).
Data for: Performance of alternative diagnostic tests; Loop Isothermal Amplification Assay (LAMP), CFW+KOH, and a Smartphone based microscope-Dipple in the diagnosis of Fungal Keratitis among Patients attending MURHEC- Southwestern Uganda.
[Dataset].
London School of Hygiene & Tropical Medicine, London, United Kingdom.
10.17037/DATA.00005249.
This dataset contains information collected from 88 patients enrolled in the study who presented at the Mbarara University and Referral Hospital Eye Hospital with signs of microbial keratitis between November 2023 and June 2025. It includes variables detailing participant demographics, clinical history, clinical presentation, and laboratory diagnoses based on corneal sample analysis.
Keywords
Microbial keratitis; Bacterial keratitis; Fungal keratitis; keratitis; Blindness; LAMP; Uganda| Item Type | Dataset |
|---|---|
| Resource Type |
Resource Type Resource Description Dataset Quantitative |
| Description of data capture | We prospectively recruited patients presenting with microbial keratitis (MK) at Mbarara University Referral Hospital and Eye Centre (MURHEC) between November 2023 and June 2025. Social demographic information was collected. Corneal scrapings were obtained from patients (n =88) with clinically suspected fungal keratitis. Routine diagnostic tests included microscopy (KOH and Gram stain), In vivo confocal microscopy (IVCM), Culture and Polymerase chain reaction (PCR). The alternative tests were Smartphone based microscope-Dipple, Loop isothermal amplification test (LAMP) and Calcofluor white+ Potassium Hydroxide (CFW+KOH). The data was analyzed with Stata 17 and summarized as tables for diagnostic yield by method, sensitivity and specificity of alternative tests against composite reference standard of IVCM, Microscopy and culture. The agreement and accuracy of the tests under study was presented by Kappa scores and McNemar’s p value. |
| Capture method | Unknown |
| Collection Period |
From To November 2023 June 2025 |
| Date | 7 July 2026 |
| Language(s) of written materials | English |
| Creator(s) |
Arunga, S; Orishaba, F; Ebong, A; Kassaza, K and Karuhanga, N
|
| Associated roles | Ayebare, D (Other); Leck, A (Other); Butcher, RMR (Other); Onyango, J (Other); Bazira, J (Other) and Burton, MJ (Other) |
| LSHTM Faculty/Department |
Faculty of Infectious and Tropical Diseases > Department of Infection Biology Faculty of Infectious and Tropical Diseases > Dept of Clinical Research |
| Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
| Funders |
Project Funder Grant Number Funder URI Performance of alternative diagnostic tests; Loop Isothermal Amplification Assay (LAMP), CFW+KOH, and a Smartphone based microscope-Dipple in the diagnosis of Fungal Keratitis among Patients attending MURHEC- Southwestern Uganda 703 https://veluxstiftung.ch/ Performance of alternative diagnostic tests; Loop Isothermal Amplification Assay (LAMP), CFW+KOH, and a Smartphone based microscope-Dipple in the diagnosis of Fungal Keratitis among Patients attending MURHEC- Southwestern Uganda 207472/Z/17/Z https://doi.org/10.13039/100010269 |
| Date Deposited | 07 Jul 2026 16:23 |
| Last Modified | 09 Jul 2026 09:42 |
| Publisher | London School of Hygiene & Tropical Medicine |
Data / Code
novel_test_dataset.zip
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subject - Data
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lock - Restricted to Request access for all
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copyright - Available under Data Sharing Agreement
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info - Data collected from patients who presented with signs of microbial keratitis
folder_zip - application/zip
- folder_info
- 333B
Documentation
novel_test_dataset_codebook.html
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subject - Documentation
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- Available under Creative Commons: Attribution 4.0
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info - Codebook for the Fungal Keratitis dataset
html - text/html
- folder_info
- 11kB
STARD-2015-Checklist.docx
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subject - Documentation
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- Available under Creative Commons: Attribution 4.0
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info - SIARD 2015 checklist for paper
description - application/vnd.openxmlformats-officedocument.wordprocessingml.document
- folder_info
- 197kB
Supplementary_material.docx
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subject - Documentation
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- Available under Creative Commons: Attribution 4.0
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info - Supplementary material file containing details on the reaction mix and master mix target concentration
description - application/vnd.openxmlformats-officedocument.wordprocessingml.document
- folder_info
- 16kB
5249_UserGuide.html
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subject - Documentation
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- Available under Creative Commons: Attribution 4.0
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info - User guide for Fungal Keratitis dataset
html - text/html
- folder_info
- 8kB
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