Replication Data for: Assessing the impact of SARS-CoV-2 spike mutations on antibody binding: comparative assessment of Wuhan and JN.1 variants’ full-length spikes in a multiplex Luminex assay

This is a replication dataset for the manuscript titled: "Assessing the impact of SARS-CoV-2 spike mutations on antibody binding: comparative assessment of Wuhan and JN.1 variants’ full-length spikes in a multiplex Luminex assay ." The SARS-CoV-2 seroprevalence estimates have been carried out by institutions under the Kenya Multi-Site sero-surveillance (KEMIS) collaboration using in house KWTRP ELISA. To determine the impact of omicron mutations on serostatus classification by ELISA assays using Wuhan spike as capture antigen, we conducted a direct comparison of Wuhan spike and JN.1 spike based assays. We directly used pre-pandemic serum/plasma collected in 2018 from 160 blood donors as gold standard negative samples. As gold standard positives wildtype, we assayed serum/plasma from 160 SARS-CoV-2 PCR-positive patients, 160 vaccination-confirmed participants with verbal confirmed infection as gold standard positive hybrids, 160 omicron confirmed positive samples as gold standard omicron positives and 400 samples with unknown status. We obtained ODs(Optical density) from the samples' reactivity to SARS-CoV-2 antigen on both ELISA assays as well as MFI (mean fluorescence intensity) in Luminex. We then expressed the OD and MFI into a ratio using the control samples. The ratios from both assays were then used to determine specificity and sensitivity. Pairwise comparisons between the two antigens was done using McNemar's test.

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