Kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut

Koussis, KORCID logo; Haase, S; Withers-Martinez, C; Flynn, HR; Kunzelmann, S; Christodoulou, E; Ibrahim, F; Skehel, M; Baker, DAORCID logo and Blackman, MJORCID logo (2024). Kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut. [Dataset]. PLOS Pathogens. https://doi.org/10.1371/journal.ppat.1012360.s018
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Dataset showing the kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut for: "Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites".

Keywords

Phosphorylation; Plasmodium; Malarial parasites; Plasmid construction; Recombinant proteins; Parasitic life cycles; In vitro kinase assay; Malaria

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