Kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut

Koussis, K

; Haase, S; Withers-Martinez, C; Flynn, HR; Kunzelmann, S; Christodoulou, E; Ibrahim, F; Skehel, M; Baker, DA

and Blackman, MJ

(2024). Kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut. [Dataset]. PLOS Pathogens. https://doi.org/10.1371/journal.ppat.1012360.s018

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Dataset showing the kinetic properties of the recombinantly expressed PfPKGWT, PfPKGY214, PfPKGSTmut, PfPKGT695A, PfPKGT695E and CD analysis of PfPKGWT and PfPKGSTmut for: "Activation loop phosphorylation and cGMP saturation of PKG regulate egress of malaria parasites".

Keywords

Phosphorylation; Plasmodium; Malarial parasites; Plasmid construction; Recombinant proteins; Parasitic life cycles; In vitro kinase assay; Malaria

Item Type	Dataset
Resource Type	Resource Type Resource Description Dataset Quantitative
Capture method	Experiment
Date	27 June 2024
Language(s) of written materials	English
Creator(s)	Koussis, K ; Haase, S; Withers-Martinez, C; Flynn, HR; Kunzelmann, S; Christodoulou, E; Ibrahim, F; Skehel, M; Baker, DA and Blackman, MJ
LSHTM Faculty/Department	Faculty of Infectious and Tropical Diseases > Department of Infection Biology
Participating Institutions	Francis Crick Institute, London, United Kingdom; London School of Hygiene & Tropical Medicine, London, United Kingdom
Funders	Project Funder Grant Number Funder URI UNSPECIFIED Wellcome Trust 220318/A/20/Z http://dx.doi.org/10.13039/100010269 UNSPECIFIED Wellcome Trust 220318/Z/20/Z http://dx.doi.org/10.13039/100010269 UNSPECIFIED Francis Crick Institute UNSPECIFIED http://dx.doi.org/10.13039/100010438
Date Deposited	18 Jul 2024 13:52
Last Modified	05 Aug 2025 13:57
Publisher	PLOS Pathogens