Patel, A, Nofal, SD, Blackman, MJ and Baker, DA. 2022. Supplementary information, CDC50 orthologues in Plasmodium falciparum have distinct roles in merozoite egress and trophozoite maturation. [Online]. Zenodo. Available from: https://doi.org/10.5281/zenodo.6619340
Patel, A, Nofal, SD, Blackman, MJ and Baker, DA. Supplementary information, CDC50 orthologues in Plasmodium falciparum have distinct roles in merozoite egress and trophozoite maturation [Internet]. Zenodo; 2022. Available from: https://doi.org/10.5281/zenodo.6619340
Patel, A, Nofal, SD, Blackman, MJ and Baker, DA (2022). Supplementary information, CDC50 orthologues in Plasmodium falciparum have distinct roles in merozoite egress and trophozoite maturation. [Data Collection]. Zenodo. https://doi.org/10.5281/zenodo.6619340
Description
In model organisms, P4-ATPases require cell division control protein 50 (CDC50) chaperones for their phospholipid flipping activity. In the malaria parasite Plasmodium falciparum, guanylyl cyclase alpha (GCα) is an integral membrane protein that is essential for release (egress) of merozoites from their host erythrocytes. GCα is unusual in that it contains both a C-terminal cyclase domain and an N-terminal P4-ATPase domain of unknown function. We sought to investigate whether any of the three CDC50 orthologues (denoted A, B and C) encoded by P. falciparum are required for GCα function. Using gene tagging and conditional gene disruption, we demonstrate that CDC50B and CDC50C but not CDC50A are expressed in the clinically important asexual blood stages and that CDC50B is a binding partner of GCα whereas CDC50C is the binding partner of another putative P4-ATPase, ATP2. Our findings indicate that CDC50B has no essential role for intraerythrocytic parasite maturation but modulates the rate of parasite egress by interacting with GCα for optimal cGMP synthesis. In contrast, CDC50C is essential for blood stage trophozoite maturation. Additionally, we find that the CDC50C-ATP2 complex may influence parasite endocytosis of host cell haemoglobin and consequently hemozoin formation.
Keywords
Data capture method | Experiment |
---|---|
Date (Date published in a 3rd party system) | 7 June 2022 |
Language(s) of written materials | English |
Data Creators | Patel, A, Nofal, SD, Blackman, MJ and Baker, DA |
---|---|
LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Department of Infection Biology |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 07 Jun 2022 10:40 |
---|---|
Last Modified | 07 Jun 2022 10:40 |
Publisher | Zenodo |