Lynham, S and Dawson, L. 2021. C. difficle p-cresol production. [Online]. ProteomeXchange. Available from: http://proteomecentral.proteomexchange.org/PXD028948
Lynham, S and Dawson, L. C. difficle p-cresol production [Internet]. ProteomeXchange; 2021. Available from: http://proteomecentral.proteomexchange.org/PXD028948
Lynham, S and Dawson, L (2021). C. difficle p-cresol production. [Data Collection]. ProteomeXchange. http://proteomecentral.proteomexchange.org/PXD028948
Description
To identify the cellular localisation of the HpdBCA decarboxylase complex, a plasmid based HpdB-SNAP-tag translational fusion was constructed using a C. difficile compatible plasmid (pMTL84151) under control of the hpdBCA promoter. The hpdB coding sequence omitting the stop codon, was fused via a linker to a SNAP-tag (Table 1). Confirmation of HpdB linked to the SNAP-tag was carried out via western blot, and mass spectrometry, in which we identified six peptides, four with 100% identity and two with 99% identity unique to HpdB. Localisation of HpdB was visualised by confocal microscopy in 630Δerm and the p-cresol deficient mutant (hpdC::CT) (carrying the HpdB-SNAP-tag fusion (PhpdB-CDS-SNAP), in the presence and absence of p-HPA) to induce HpdBCA production.
Keywords
Data capture method | Experiment: Laboratory |
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Date (Date published in a 3rd party system) | 2 November 2021 |
Language(s) of written materials | English |
Data Creators | Lynham, S and Dawson, L |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Dept of Clinical Research Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 26 Nov 2021 12:34 |
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Last Modified | 26 Nov 2021 12:36 |
Publisher | ProteomeXchange |