London School of Hygiene & Tropical Medicine. 2019. Transgenic P. knowlesi orthologue replacement lines provide surrogates for P. vivax vaccine development and Duffy Binding Protein tropism studies. [Online]. European Nucleotide Archive. Available from: https://www.ebi.ac.uk/ena/data/view/PRJEB30925
London School of Hygiene & Tropical Medicine. Transgenic P. knowlesi orthologue replacement lines provide surrogates for P. vivax vaccine development and Duffy Binding Protein tropism studies [Internet]. European Nucleotide Archive; 2019. Available from: https://www.ebi.ac.uk/ena/data/view/PRJEB30925
London School of Hygiene & Tropical Medicine (2019). Transgenic P. knowlesi orthologue replacement lines provide surrogates for P. vivax vaccine development and Duffy Binding Protein tropism studies. [Data Collection]. European Nucleotide Archive. https://www.ebi.ac.uk/ena/data/view/PRJEB30925
Alternative Title
PRJEB30925
Description
Tackling relapsing Plasmodium vivax and zoonotic Plasmodium knowlesi infections is critical to reducing malaria incidence and mortality worldwide. Understanding the biology of these important and related parasites was previously constrained by the lack of robust molecular and genetic approaches, particularly for P. vivax. Here, we establish CRISPR-Cas9 genome editing in a culture-adapted strain of P. knowlesi and define critical parameters for optimal homology-driven repair. Using iterative rounds of genome editing of the P. knowlesi Duffy binding protein family genes we generate a transgenic line expressing P. vivax Duffy binding protein (PvDBP), a lead vaccine candidate. We demonstrate that PvDBP plays no role in reticulocyte restriction but can alter the macaque/human host cell tropism of P. knowlesi. Critically, antibodies raised against the P. vivax antigen potently inhibit proliferation of this strain, providing an invaluable tool to support vaccine development.
Additional information
Secondary study ID: ERP113417
Keywords
Data capture method | Experiment |
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Date (Date published in a 3rd party system) | 22 January 2019 |
Language(s) of written materials | English |
Data Creators | London School of Hygiene & Tropical Medicine |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Administration |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 01 May 2020 11:20 |
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Last Modified | 01 May 2020 11:20 |
Publisher | European Nucleotide Archive |