Hierarchical genomic analysis of carried and invasive serogroup A Neisseria meningitidis during the 2011 epidemic in Chad

Harvard | Vancouver

Diallo, K, Gamougam, K, Daugla, DM, Harrison, OB, Bray, JE, Caugant, DA, Lucidarme, J, Trotter, CL, Hassan-King, M, Stuart, JM, Manigart, O, Greenwood, BM and Maiden, MCJ. 2017. Hierarchical genomic analysis of carried and invasive serogroup A Neisseria meningitidis during the 2011 epidemic in Chad. [Online]. Figshare. Available from: - https://doi.org/10.6084/m9.figshare.c.3785438

Export Citation

Sharing

Description

Serogroup A Neisseria meningitidis (NmA) was the cause of the 2011 meningitis epidemics in Chad. This bacterium, often carried asymptomatically, is considered to be an “accidental pathogen”; however, the transition from carriage to disease phenotype remains poorly understood. This study examined the role genetic diversity might play in this transition by comparing genomes from geographically and temporally matched invasive and carried NmA isolates. Results All 23 NmA isolates belonged to the ST-5 clonal complex (cc5). Ribosomal MLST comparison with other publically available NmA:cc5 showed that isolates were closely related, although those from Chad formed two distinct branches and did not cluster with other NmA, based on their MLST profile, geographical and temporal location. Whole genome MLST (wgMLST) comparison identified 242 variable genes among all Chadian isolates and clustered them into three distinct phylogenetic groups (Clusters 1, 2, and 3): no systematic clustering by disease or carriage source was observed. There was a significant difference (p = 0.0070) between the mean age of the individuals from which isolates from Cluster 1 and Cluster 2 were obtained, irrespective of whether the person was a case or a carrier. Conclusions Whole genome sequencing provided high-resolution characterization of the genetic diversity of these closely related NmA isolates. The invasive meningococcal isolates obtained during the epidemic were not homogeneous; rather, a variety of closely related but distinct clones were circulating in the human population with some clones preferentially colonizing specific age groups, reflecting a potential age-related niche adaptation. Systematic genetic differences were not identified between carriage and disease isolates consistent with invasive meningococcal disease being a multi-factorial event resulting from changes in host-pathogen interactions along with the bacterium.

Published in a 3rd party system Date: 12 June 2017
Data capture method:
Mode of data capture
Experiment
Data Creators(s): Diallo, K, Gamougam, K, Daugla, DM, Harrison, OB, Bray, JE, Caugant, DA, Lucidarme, J, Trotter, CL, Hassan-King, M, Stuart, JM, Manigart, O, Greenwood, BM and Maiden, MCJ
LSHTM Faculty/Department: Faculty of Infectious and Tropical Diseases > Dept of Disease Control
Participating Institutions: London School of Hygiene & Tropical Medicine

Files

Data not available from this repository.

Dataset may be available externally from:

Related resources

LSHTM Open Access publications:

Resources

Actions

Edit Item Edit Item (admin only)