High Throughput Screening Identifies Novel Lead Compounds with Activity against Larval, Juvenile and Adult Schistosoma mansoni

Harvard | Vancouver

Mansour, NR, Paveley, R, Gardner, JMF, Bell, AS, Parkinson, T and Bickle, Q. 2016. High Throughput Screening Identifies Novel Lead Compounds with Activity against Larval, Juvenile and Adult Schistosoma mansoni. [Online]. Figshare. Available from: 10.1371/journal.pntd.0004659

Export Citation

Sharing

Description

An estimated 600 million people are affected by the helminth disease schistosomiasis caused by parasites of the genus Schistosoma. There is currently only one drug recommended for treating schistosomiasis, praziquantel (PZQ), which is effective against adult worms but not against the juvenile stage. In an attempt to identify improved drugs for treating the disease, we have carried out high throughput screening of a number of small molecule libraries with the aim of identifying lead compounds with balanced activity against all life stages of Schistosoma. A total of almost 300,000 compounds were screened using a high throughput assay based on motility of worm larvae and image analysis of assay plates. Hits were screened against juvenile and adult worms to identify broadly active compounds and against a mammalian cell line to assess cytotoxicity. A number of compounds were identified as promising leads for further chemical optimization.

Published in a 3rd party system Date: 29 April 2016
Data capture method:
Mode of data capture
Experiment
Data Creators(s): Mansour, NR, Paveley, R, Gardner, JMF, Bell, AS, Parkinson, T and Bickle, Q
LSHTM Faculty/Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Participating Institutions: Study consortium

Files

Data

Resources

Actions

Edit Item Edit Item (admin only)