Shigella induces epigenetic reprogramming of zebrafish neutrophils

Gomes, MC; Brokatzky, D

; Wardle, FC and Mostowy, S

(2023). Shigella induces epigenetic reprogramming of zebrafish neutrophils. [Dataset]. NCBI Gene Expression Omnibus. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063

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The ChIP-seq data of neutrophils 48 hours after Shigella training for: "Shigella induces epigenetic reprogramming of zebrafish neutrophils".

Keywords

Mitochondria; Neutrophils; Shigella flexneri; Trained Immunity; Zebrafish

Item Type	Dataset
Resource Type	Resource Type Resource Description Dataset Quantitative
Description of data capture	Trained immunity is a long-term memory of innate immune cells, generating an improved response upon re-infection. Shigella is an important human pathogen and inflammatory paradigm for which there is no effective vaccine. Using zebrafish larvae we demonstrate that after Shigella priming neutrophils are more efficient at bacterial clearance. We observe that Shigella-induced protection is non-specific and long-lasting, and is unlike training by BCG and β-glucan. Analysis of histone ChIP-seq on primed neutrophils revealed that Shigella training deposits the active H3K4me3 mark on promoter regions of 1612 genes, significantly changing the epigenetic landscape of neutrophils towards enhanced microbial recognition and mitochondrial ROS production. Finally, we demonstrate that mitochondrial ROS plays a key role in enhanced antimicrobial activity of trained neutrophils. It is envisioned that signals and mechanisms we discover here can be used in other vertebrates, including humans, to suggest new therapeutic strategies involving neutrophils to control bacterial infection.
Capture method	Unknown
Date	13 September 2023
Language(s) of written materials	English
Creator(s)	Gomes, MC; Brokatzky, D ; Bielecka, MK ; Wardle, FC and Mostowy, S
LSHTM Faculty/Department	Faculty of Infectious and Tropical Diseases > Department of Infection Biology
Participating Institutions	London School of Hygiene & Tropical Medicine, London, United Kingdom; King's College London, London, United Kingdom
Funders	Project Funder Grant Number Funder URI UNSPECIFIED DFG Walter Benjamin Programme BR 6637/1-1 UNSPECIFIED UNSPECIFIED Biotechnology and Biological Sciences Research Council BB/R016283/ http://dx.doi.org/10.13039/501100000268 Consolidator Grant European Research Council 772853–ENTRAPMENT http://dx.doi.org/10.13039/501100000781 Senior Research Fellowship Wellcome Trust 206444/Z/17/Z http://dx.doi.org/10.13039/100010269 UNSPECIFIED Lister Institute of Preventive Medicine UNSPECIFIED http://dx.doi.org/10.13039/501100001255
Date Deposited	16 Feb 2024 09:47
Last Modified	28 Aug 2025 12:51
Publisher	NCBI Gene Expression Omnibus