Shigella induces epigenetic reprogramming of zebrafish neutrophils

Gomes, MC, Brokatzky, D, Bielecka, MK, Wardle, FC and Mostowy, S. 2023. Shigella induces epigenetic reprogramming of zebrafish neutrophils. [Online]. NCBI Gene Expression Omnibus. Available from: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063

Gomes, MC, Brokatzky, D, Bielecka, MK, Wardle, FC and Mostowy, S. Shigella induces epigenetic reprogramming of zebrafish neutrophils [Internet]. NCBI Gene Expression Omnibus; 2023. Available from: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063

Gomes, MC, Brokatzky, D, Bielecka, MK, Wardle, FC and Mostowy, S (2023). Shigella induces epigenetic reprogramming of zebrafish neutrophils. [Data Collection]. NCBI Gene Expression Omnibus. https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE217063

Description

Description of data capture Trained immunity is a long-term memory of innate immune cells, generating an improved response upon re-infection. Shigella is an important human pathogen and inflammatory paradigm for which there is no effective vaccine. Using zebrafish larvae we demonstrate that after Shigella priming neutrophils are more efficient at bacterial clearance. We observe that Shigella-induced protection is non-specific and long-lasting, and is unlike training by BCG and β-glucan. Analysis of histone ChIP-seq on primed neutrophils revealed that Shigella training deposits the active H3K4me3 mark on promoter regions of 1612 genes, significantly changing the epigenetic landscape of neutrophils towards enhanced microbial recognition and mitochondrial ROS production. Finally, we demonstrate that mitochondrial ROS plays a key role in enhanced antimicrobial activity of trained neutrophils. It is envisioned that signals and mechanisms we discover here can be used in other vertebrates, including humans, to suggest new therapeutic strategies involving neutrophils to control bacterial infection.
Data capture method Unknown
Date (Date published in a 3rd party system) 13 September 2023
Language(s) of written materials English
Data Creators Gomes, MC, Brokatzky, D, Bielecka, MK, Wardle, FC and Mostowy, S
LSHTM Faculty/Department Faculty of Infectious and Tropical Diseases > Department of Infection Biology
Participating Institutions London School of Hygiene & Tropical Medicine, London, United Kingdom, King's College London, London, United Kingdom
Funders
ProjectFunderGrant NumberFunder URI
UNSPECIFIEDDFG Walter Benjamin ProgrammeBR 6637/1-1UNSPECIFIED
UNSPECIFIEDBiotechnology and Biological Sciences Research CouncilBB/R016283/http://dx.doi.org/10.13039/501100000268
Consolidator GrantEuropean Research Council772853–ENTRAPMENThttp://dx.doi.org/10.13039/501100000781
Senior Research FellowshipWellcome Trust206444/Z/17/Zhttp://dx.doi.org/10.13039/100010269
UNSPECIFIEDLister Institute of Preventive MedicineUNSPECIFIEDhttp://dx.doi.org/10.13039/501100001255
Date Deposited 16 Feb 2024 09:47
Last Modified 16 Feb 2024 09:47
Publisher NCBI Gene Expression Omnibus

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