Kutima, B, Kagucia, W, Mwai, K, Kimani, M, Sigilai, A, Mugo, D, Karani, K, Akech, D, Toroitich, M, Karia, B, Tuju, J, Ziraba, AK, Bigogo, G, Ochieng, C, Onyango, C, Lidechi, S, Munywoki, PK, Uyoga, S, Adetifa, IMO, Ochola-Oyier, LI, Bejon, P, Scott, JAG, Agweyu, A, Warimwe, GM and Nyagwange, J. 2023. Replication Data for: Comparative performance of the InBios SCoV-2 DetectTM IgG ELISA and the in-house KWTRP ELISA in detecting SARS-CoV-2 spike IgG antibodies in Kenyan populations. [Online]. Harvard Dataverse. Available from: https://doi.org/10.7910/DVN/FOYVMT
Kutima, B, Kagucia, W, Mwai, K, Kimani, M, Sigilai, A, Mugo, D, Karani, K, Akech, D, Toroitich, M, Karia, B, Tuju, J, Ziraba, AK, Bigogo, G, Ochieng, C, Onyango, C, Lidechi, S, Munywoki, PK, Uyoga, S, Adetifa, IMO, Ochola-Oyier, LI, Bejon, P, Scott, JAG, Agweyu, A, Warimwe, GM and Nyagwange, J. Replication Data for: Comparative performance of the InBios SCoV-2 DetectTM IgG ELISA and the in-house KWTRP ELISA in detecting SARS-CoV-2 spike IgG antibodies in Kenyan populations [Internet]. Harvard Dataverse; 2023. Available from: https://doi.org/10.7910/DVN/FOYVMT
Kutima, B, Kagucia, W, Mwai, K, Kimani, M, Sigilai, A, Mugo, D, Karani, K, Akech, D, Toroitich, M, Karia, B, Tuju, J, Ziraba, AK, Bigogo, G, Ochieng, C, Onyango, C, Lidechi, S, Munywoki, PK, Uyoga, S, Adetifa, IMO, Ochola-Oyier, LI, Bejon, P, Scott, JAG, Agweyu, A, Warimwe, GM and Nyagwange, J (2023). Replication Data for: Comparative performance of the InBios SCoV-2 DetectTM IgG ELISA and the in-house KWTRP ELISA in detecting SARS-CoV-2 spike IgG antibodies in Kenyan populations. [Data Collection]. Harvard Dataverse. https://doi.org/10.7910/DVN/FOYVMT
Description
This is a replication dataset for the manuscript titled "Comparative performance of the InBios SCoV-2 DetectTM IgG ELISA and the in-house KWTRP ELISA in detecting SARS-CoV-2 spike IgG antibodies in Kenyan populations." The SARS-CoV-2 seroprevalence estimates have been carried out by institutions under the Kenya Multi-Site Sero-surveillance (KEMIS) collaboration using both the InBios SCoV-2 DetectTM IgG ELISA and in house KWTRP ELISA. For the comparability of data collected using both tests by KEMIS participating sites, we conducted a direct comparison of these assays. We directly used pre-pandemic serum/plasma collected in 2018 from 454 blood donors and 173 malaria cross-sectional survey participants designated gold standard negatives. As gold standard positives, we assayed serum/plasma from 159 SARS-CoV-2 PCR-positive patients and 166 vaccination-confirmed participants.We obtained ODs(Optical density) from the samples' reactivity to SARS-CoV-2 antigen on both ELISA assays. We then expressed the OD into a ratio using the control samples. The ratios from both assays were then used to determine specificity, sensitivity, and prevalence. Pairwise comparisons between the InBios and KWTRP was done and assays’ reproducibility was assesed by examining the raw ODs and coefficient of variation (CV) for the negative, positive and cut-off controls.
Keywords
Data capture method | Measurements and tests |
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Date (Date published in a 3rd party system) | 10 August 2023 |
Language(s) of written materials | English |
Data Creators | Kutima, B, Kagucia, W, Mwai, K, Kimani, M, Sigilai, A, Mugo, D, Karani, K, Akech, D, Toroitich, M, Karia, B, Tuju, J, Ziraba, AK, Bigogo, G, Ochieng, C, Onyango, C, Lidechi, S, Munywoki, PK, Uyoga, S, Adetifa, IMO, Ochola-Oyier, LI, Bejon, P, Scott, JAG, Agweyu, A, Warimwe, GM and Nyagwange, J |
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Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
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Date Deposited | 29 Jan 2024 17:11 |
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Last Modified | 29 Jan 2024 17:11 |
Publisher | Harvard Dataverse |