Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis

Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG. 2022. Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis. [Online]. London School of Hygiene & Tropical Medicine, London, United Kingdom. Available from: https://doi.org/10.17037/DATA.00003114.

Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG. Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis [Internet]. London School of Hygiene & Tropical Medicine; 2022. Available from: https://doi.org/10.17037/DATA.00003114.

Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG (2022). Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis. [Data Collection]. London School of Hygiene & Tropical Medicine, London, United Kingdom. https://doi.org/10.17037/DATA.00003114.

Description

Description of data capture Field data: Per household, fly contacts on two children aged 2-9 years were observed for ten minutes. If children were unavailable or there were not two children aged 2-9 years, children aged >9 years were observed (with a preference for the youngest), if no children were present adults were observed. Fly contact observations were made outside in a shady area, and two entomological field workers tallied fly-eye, -nose and -mouth contacts. The face was filmed (using a tripod-mounted Nikon D7200 single-lens reflex camera) for the 10-min observation period. Flies landing on individual’s faces were caught, killed, and transported on ice to our local laboratory as previously described. The observed child sat on a chair and for 15 minutes, or until 10 nets were used, flies landing on the child’s face were disturbed and caught in a sterile net. In the laboratory, flies were identified with keys as M. sorbens, M. domestica, ‘other’ (clearly another species) or ‘unknown’ (unidentifiable) using dissecting microscopes. Musca sorbens and M. domestica were sexed; flies were temporarily stored at -20˚C before transfer to a -80˚C freezer, where they were stored until DNA extraction and testing. Laboratory data: Internal and external recovery of Ct DNA was tested using six female flies (experiment 1), six male flies (experiment 2), and six males/six females (experiment 3). Musca sorbens (broad frons), colonised in 2018, were reared at the London School of Hygiene & Medicine according to previously published methods. Flies of known and uniform age were starved of food (milk/sugar) and water prior to feeding experiments. After starving, surviving flies were put into individual containers covered by a square of mesh with a small hole in the middle plugged with cotton wool. In a microbiological safety cabinet, flies were fed aliquots of Chlamydia trachomatis culture (culture lysate containing EB at approximately 1000 EB/uL) by pipette through the hole in the mesh. All samples were stored immediately in sterile tubes at -20oC. DNA extraction, PCR quantification and load estimation was performed as for the field samples.
Data capture method Field observation: Participant, Lab observation: Non-participant
Data Collection Period
FromTo
April 2018January 2022
Date (Date submitted to LSHTM repository) 14 October 2022
Geographical area covered (offline during plugin upgrade)
North LatitudeEast LongitudeSouth LatitudeWest Longitude
7.3103838.8087.0562738.4585
51.5365-0.022199951.4476-0.2227
Language(s) of written materials English
Data Creators Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG
LSHTM Faculty/Department Faculty of Epidemiology and Population Health > Dept of Infectious Disease Epidemiology
Faculty of Infectious and Tropical Diseases > Dept of Clinical Research
Faculty of Infectious and Tropical Diseases > Dept of Disease Control
Participating Institutions London School of Hygiene & Tropical Medicine, London, United Kingdom, Natural History Museum, London, United Kingdom, Fred Hollows Foundation, Addis Ababa, Ethiopia
Funders
ProjectFunderGrant NumberFunder URI
Stronger SAFEWellcome TrustUNSPECIFIEDhttp://dx.doi.org/10.13039/100010269
Stronger SAFEFred Hollows FoundationUNSPECIFIEDUNSPECIFIED
Date Deposited 13 Oct 2022 17:06
Last Modified 13 Oct 2022 17:07
Publisher London School of Hygiene & Tropical Medicine

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