Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis
Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG.
2022.
Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis.
[Online].
London School of Hygiene & Tropical Medicine, London, United Kingdom.
Available from:
https://doi.org/10.17037/DATA.00003114.
Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG.
Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis
[Internet].
London School of Hygiene & Tropical Medicine;
2022.
Available from:
https://doi.org/10.17037/DATA.00003114.
Robinson, A, Versteeg, B, Shafi Abdurahman, O, Clatworthy, I, Shuka, G, Debe, D, Hordofa, G, Reis de Oliveira Gomes, L, Abraham Aga, M, Dumessa, G, Sarah, V, Macleod, D, Last, AR, Burton, MJ and Logan, JG
(2022).
Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis.
[Data Collection].
London School of Hygiene & Tropical Medicine, London, United Kingdom.
https://doi.org/10.17037/DATA.00003114.
Description
A population-based survey of 247 households was conducted in Shashemane district, Oromia Region, Ethiopia, between April and June 2018. Households were positively selected to include at least one child aged 1–9 years, resident on the day of enumeration. These datasets contain entomology from this survey; including fly-eye contact data, fly positivity for Chlamydia trachomatis and other entomological data (fly species, sex). Experiments were also conducted in the London School of Hygiene & Tropical Medicine to investigate transmission of Chlamydia trachomatis by Musca sorbens, those data are reported here.
Field data: Per household, fly contacts on two children aged 2-9 years were observed for ten minutes. If children were unavailable or there were not two children aged 2-9 years, children aged >9 years were observed (with a preference for the youngest), if no children were present adults were observed. Fly contact observations were made outside in a shady area, and two entomological field workers tallied fly-eye, -nose and -mouth contacts. The face was filmed (using a tripod-mounted Nikon D7200 single-lens reflex camera) for the 10-min observation period. Flies landing on individual’s faces were caught, killed, and transported on ice to our local laboratory as previously described. The observed child sat on a chair and for 15 minutes, or until 10 nets were used, flies landing on the child’s face were disturbed and caught in a sterile net. In the laboratory, flies were identified with keys as M. sorbens, M. domestica, ‘other’ (clearly another species) or ‘unknown’ (unidentifiable) using dissecting microscopes. Musca sorbens and M. domestica were sexed; flies were temporarily stored at -20˚C before transfer to a -80˚C freezer, where they were stored until DNA extraction and testing.
Laboratory data: Internal and external recovery of Ct DNA was tested using six female flies (experiment 1), six male flies (experiment 2), and six males/six females (experiment 3). Musca sorbens (broad frons), colonised in 2018, were reared at the London School of Hygiene & Medicine according to previously published methods. Flies of known and uniform age were starved of food (milk/sugar) and water prior to feeding experiments. After starving, surviving flies were put into individual containers covered by a square of mesh with a small hole in the middle plugged with cotton wool. In a microbiological safety cabinet, flies were fed aliquots of Chlamydia trachomatis culture (culture lysate containing EB at approximately 1000 EB/uL) by pipette through the hole in the mesh. All samples were stored immediately in sterile tubes at -20oC. DNA extraction, PCR quantification and load estimation was performed as for the field samples.
London School of Hygiene & Tropical Medicine, London, United Kingdom, Natural History Museum, London, United Kingdom, Fred Hollows Foundation, Addis Ababa, Ethiopia
Description: Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis
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