Barry, A, Behet, MC, Nébié, I, Lanke, K, Grignard, L, Ouedraogo, A, Soulama, I, Drakeley, C, Sauerwein, R, Bolscher, JM, Dechering, KJ, Bousema, T, Tiono, AB and Gonçalves, BP. 2019. Data from: Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso. [Online]. Dryad. Available from: https://doi.org/10.5061/dryad.n1m33qq
Barry, A, Behet, MC, Nébié, I, Lanke, K, Grignard, L, Ouedraogo, A, Soulama, I, Drakeley, C, Sauerwein, R, Bolscher, JM, Dechering, KJ, Bousema, T, Tiono, AB and Gonçalves, BP. Data from: Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso [Internet]. Dryad; 2019. Available from: https://doi.org/10.5061/dryad.n1m33qq
Barry, A, Behet, MC, Nébié, I, Lanke, K, Grignard, L, Ouedraogo, A, Soulama, I, Drakeley, C, Sauerwein, R, Bolscher, JM, Dechering, KJ, Bousema, T, Tiono, AB and Gonçalves, BP (2019). Data from: Functional antibodies against Plasmodium falciparum sporozoites are associated with a longer time to qPCR-detected infection among schoolchildren in Burkina Faso. [Data Collection]. Dryad. https://doi.org/10.5061/dryad.n1m33qq
Description
Background: Individuals living in malaria-endemic regions develop naturally acquired immunity against severe malarial disease, but it is unclear whether immunity that affects the establishment of infections develops following continuous natural exposure. Methods: We cleared schoolchildren in Burkina Faso of possible sub-patent infections and examined them weekly for incident infections by PCR. Plasma samples collected at enrolment were used to quantify antibodies to the pre-eryhrocytic-stage antigens circumsporozoite protein (CSP) and liver stage antigen. Sporozoite gliding inhibition by naturally acquired antibodies was assessed using Plasmodium falciparum NF54 sporozoites; hepatocyte invasion was assessed using the human HC-04 hepatoma cell line and NF54 sporozoites. The associations between these functional pre-erythrocytic immunity phenotypes and time to PCR-detected infection were studied. Results: A total of 51 children were monitored; the median time to first detection of infection by PCR or development of clinical symptoms was 28 days. Anti-CSP antibody titres showed a strong positive association with sporozoite gliding motility inhibition (P<0.0001, Spearman’s ρ=0.76). In vitro hepatocyte invasion was inhibited by naturally acquired antibodies (median invasion inhibition, 19.4% [IQR 15.2-40.9%]), and there was a positive correlation between gliding and invasion inhibition (P=0.02, Spearman’s ρ=0.60). Survival analysis indicated longer time to infection in individuals displaying higher-than-median sporozoite gliding inhibition activity (P=0.01). Conclusions: In summary, functional antibodies against the pre-erythrocytic stages of malaria infection are acquired in children who are repeatedly exposed to Plasmodium parasites. This immune response does not prevent them from becoming infected during a malaria transmission season, but might delay the appearance of blood stage parasitaemia and consequently needs to be considered in the evaluation of malaria vaccines.
Keywords
Data capture method | Unknown |
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Date (Date published in a 3rd party system) | 23 April 2019 |
Language(s) of written materials | English |
Data Creators | Barry, A, Behet, MC, Nébié, I, Lanke, K, Grignard, L, Ouedraogo, A, Soulama, I, Drakeley, C, Sauerwein, R, Bolscher, JM, Dechering, KJ, Bousema, T, Tiono, AB and Gonçalves, BP |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 10 Jun 2021 09:55 |
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Last Modified | 07 Sep 2021 16:15 |
Publisher | Dryad |