Ouédraogo, AL, Bousema, T, Schneider, P, de Vlas, SJ, Ilboudo-Sanogo, E, Cuzin-Ouattara, N, Nébié, I, Roeffen, W, Verhave, JP, Luty, AJF and Sauerwein, R. 2013. Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. [Online]. Dryad. Available from: https://doi.org/10.5061/dryad.hv01f
Ouédraogo, AL, Bousema, T, Schneider, P, de Vlas, SJ, Ilboudo-Sanogo, E, Cuzin-Ouattara, N, Nébié, I, Roeffen, W, Verhave, JP, Luty, AJF and Sauerwein, R. Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission [Internet]. Dryad; 2013. Available from: https://doi.org/10.5061/dryad.hv01f
Ouédraogo, AL, Bousema, T, Schneider, P, de Vlas, SJ, Ilboudo-Sanogo, E, Cuzin-Ouattara, N, Nébié, I, Roeffen, W, Verhave, JP, Luty, AJF and Sauerwein, R (2013). Data from: Substantial contribution of submicroscopical Plasmodium falciparum gametocyte carriage to the infectious reservoir in an area of seasonal transmission. [Data Collection]. Dryad. https://doi.org/10.5061/dryad.hv01f
Description
Background: Man to mosquito transmission of malaria depends on the presence of the sexual stage parasites, gametocytes, that often circulate at low densities. Gametocyte densities below the microscopical threshold of detection may be sufficient to infect mosquitoes but the importance of submicroscopical gametocyte carriage in different transmission settings is unknown. Methodology/Principal Findings: Membrane feeding experiments were carried out on 80 children below 14 years of age at the end of the wet season in an area of seasonal malaria transmission in Burkina Faso. Gametocytes were quantified by microscopy and by Pfs25-based quantitative nucleic acid sequence-based amplification assay (QT-NASBA). The children's infectiousness was determined by membrane feeding experiments in which a venous blood sample was offered to locally reared Anopheles mosquitoes. Gametocytes were detected in 30.0% (24/80) of the children by microscopy compared to 91.6% (65/71) by QT-NASBA (p<0.001). We observed a strong association between QT-NASBA gametocyte density and infection rates (p = 0.007). Children with microscopically detectable gametocytes were more likely to be infectious (68.2% compared to 31.7% of carriers of submicroscopical gametocytes, p = 0.001), and on average infected more mosquitoes (13.2% compared to 2.3%, p<0.001). However, because of the high prevalence of submicroscopical gametocyte carriage in the study population, carriers of sub-microscopical gametocytes were responsible for 24.2% of the malaria transmission in this population. Conclusions/Significance: Submicroscopical gametocyte carriage is common in an area of seasonal transmission in Burkina Faso and contributes substantially to the human infectious reservoir. Submicroscopical gametocyte carriage should therefore be considered when implementing interventions that aim to reduce malaria transmission.
Data capture method | Measurements and tests |
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Date (Date published in a 3rd party system) | 15 May 2013 |
Language(s) of written materials | English |
Data Creators | Ouédraogo, AL, Bousema, T, Schneider, P, de Vlas, SJ, Ilboudo-Sanogo, E, Cuzin-Ouattara, N, Nébié, I, Roeffen, W, Verhave, JP, Luty, AJF and Sauerwein, R |
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LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases |
Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom |
Date Deposited | 28 Aug 2020 08:44 |
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Last Modified | 07 Sep 2021 16:14 |
Publisher | Dryad |