Phylogenetic position of Leishmania isolates from Khyber Pakhtunkhwa province of Pakistan

Harvard | Vancouver

Khan, NH, Messenger, LA, Wahid, S and Sutherland, CJ. 2016. Phylogenetic position of Leishmania isolates from Khyber Pakhtunkhwa province of Pakistan. [Online]. Experimental Parasitology. Available from: 10.1016/j.exppara.2016.05.006

Export Citation

Sharing

Description

Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmaniatropica, 3 Leishmaniainfantum), in-relation to species from other geographical areas using gene sequences encoding cytochrome b (cytb) and internal transcribed spacer 2 (its2). Based on cytochrome b sequence analysis, L. tropica strains from Pakistan and other geographical regions were differentiated into two genotype groups, A and B. Within the province, five distinct L. tropica genotypes were recognized; two in group A, three in group B. Two L. infantum isolates from the province were closely associated with both Afro-Eurasian and American species of the Leishmaniadonovani complex, including Leishmaniachagasi, L. infantum and L. donovani from Sudan and Ethiopia; while a third L. infantum isolate could not be differentiated from visceralizing Kenyan and Indian L. donovani. We observed apposite phylogenetic placement of CL-causing L. tropica and L. infantum from Khyber Pakhtunkhwa. Affinities ascribed to Leishmania spp. From the region are valuable in tracing potential importation of leishmaniasis.

Published in a 3rd party system Date: 24 May 2016
Data capture method:
Mode of data capture
Experiment: Field Intervention
Data Creators(s): Khan, NH, Messenger, LA, Wahid, S and Sutherland, CJ
LSHTM Faculty/Department: Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection
Faculty of Infectious and Tropical Diseases > Dept of Pathogen Molecular Biology
Participating Institutions: London School of Hygiene & Tropical Medicine, University of Peshawa

Files

Data

Related resources

LSHTM Open Access publications:

Resources

Actions

Edit Item Edit Item (admin only)