Collett, CF, Kitson, C, Baker, N, Steele-stallard, HB, Santrot, MV, Hutchinson, S, Horn, D and Alsford, S. 2019. Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei. [Online]. European Nucleotide Archive. Available from: https://www.ebi.ac.uk/ena/data/view/PRJEB31973
Collett, CF, Kitson, C, Baker, N, Steele-stallard, HB, Santrot, MV, Hutchinson, S, Horn, D and Alsford, S. Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei [Internet]. European Nucleotide Archive; 2019. Available from: https://www.ebi.ac.uk/ena/data/view/PRJEB31973
Collett, CF, Kitson, C, Baker, N, Steele-stallard, HB, Santrot, MV, Hutchinson, S, Horn, D and Alsford, S (2019). Chemogenomic profiling of anti-leishmanial efficacy and resistance in the related kinetoplastid parasite Trypanosoma brucei. [Data Collection]. European Nucleotide Archive. https://www.ebi.ac.uk/ena/data/view/PRJEB31973
Alternative Title
PRJEB31973
Description
The arsenal of drugs used to treat leishmaniasis, caused by Leishmania spp., is limited and beset by emergent resistance and toxicity. Our understanding of drug mode-of-action and potential routes to resistance is also limited. Forward genetic approaches have revolutionised our understanding of drug mode-of-action in the related kinetoplastid parasite, Trypanosoma brucei. Therefore, we screened our genome-scale T. brucei RNAi library for knockdowns that render cells resistant to the current anti-leishmanial drugs, sodium stibogluconate (antimonial), paromomycin, miltefosine and amphotericin-B. Identification of T. brucei orthologues of the known Leishmania antimonial and miltefosine plasma membrane transporters effectively validated our approach, while a cohort of 42 novel drug efficacy determinants provides new insights and serves as a resource.
Additional information
Secondary ID: ERP114591
Keywords
| Data capture method | Experiment |
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| Date (Date published in a 3rd party system) | April 2019 |
| Language(s) of written materials | English |
| Data Creators | Collett, CF, Kitson, C, Baker, N, Steele-stallard, HB, Santrot, MV, Hutchinson, S, Horn, D and Alsford, S |
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| LSHTM Faculty/Department | Faculty of Infectious and Tropical Diseases > Dept of Immunology and Infection |
| Participating Institutions | London School of Hygiene & Tropical Medicine, London, United Kingdom, University of Dundee, Dundee, United Kingdom. |
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| Date Deposited | 13 May 2019 16:16 |
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| Last Modified | 08 Jul 2021 12:52 |
| Publisher | European Nucleotide Archive |
