The movement of Anopheles stephensi mosquitoes into the horn of Africa has vast implications for malaria control in the region. A proficient vector of both Plasmodium falciparum and Plasmodium vivax, the presence of this vector in neighboring countries has seen significant increases in malaria cases. However, very little is known about the genomics of this invasive vector population. Here we applied amplicon sequencing technology to SDA500 colony samples and field samples collected from Ethiopia of An. stephensi. The amplicons targeted 11 regions in 6 genes. Four of these genes are involved in insecticide resistance(VGSC, ACE1, rdl, and GSTe2), and two (COI and ITS2) used for population genetics.