The aim of this study was to assess the characteristics and business practices of e-pharmacies, and their compliance with global best practice guidelines and national regulatory requirements. Study staff reviewed the websites and apps of all e-pharmacies serving retail customers in two Low and Middle Income Countries (LMICs): India, which has a very large e-pharmacy market, but is yet to enact formal e-pharmacy regulations, and Kenya, which has a smaller market, but with developed regulations. All data used in this analysis was available in the public domain at the time of data collection.