This study characterized P. malariae isolates from malaria cases across seven countries in sub-Saharan Africa, using targeted amplicon sequencing to explore polymorphisms in orthologues of P. falciparum antimalarial resistance genes (Pmaat1, Pmap2mu, Pmatp4, Pmatp6, Pmcytb, Pmdhfr, Pmdhps, Pmnhe, Pmkelch13, Pmcrt and Pmmdr1).