3114 25 13005 2 71182 document 13005 Datasets.zip application/zip 2dc721414d8e11fba21e7151272683b17903898907dfa63f83958f930fac174a SHA256 378528 2022-10-13 16:23:50 https://datacompass.lshtm.ac.uk/id/eprint/3114/1/Datasets.zip 3114 1 1 application/zip Dataset en public cc_by_4

Datasets.zip

data indefinite Field entomology data from Ethiopian trachoma population survey and laboratory transmission experiments for Musca sorbens and Chlamydia trachomatis 13006 2 71184 document 13006 3114_Codebook.html text/html 25ee3e43432277941bdc48b68a70d10ee65e31ac04f2543b19e45c6ce710edcc SHA256 41180 2022-10-13 17:03:20 https://datacompass.lshtm.ac.uk/id/eprint/3114/2/3114_Codebook.html 3114 2 2 text/html Text en public cc_by_4

3114_Codebook.html

documentation indefinite Codebook for Field entomology data 17081 1 95407 document 17081 indexcodes.txt text/plain 388661ab14048327c1bfd76472720b56995044f96aad7a6838b0f95b148072e8 SHA256 2924 2025-02-07 10:21:44 https://datacompass.lshtm.ac.uk/id/eprint/3114/3/indexcodes.txt 3114 3 3 text/plain en public

indexcodes.txt

http://eprints.org/relation/isVersionOf https://datacompass.lshtm.ac.uk/id/document/13006 http://eprints.org/relation/isVolatileVersionOf https://datacompass.lshtm.ac.uk/id/document/13006 http://eprints.org/relation/isIndexCodesVersionOf https://datacompass.lshtm.ac.uk/id/document/13006 indefinite Generate index codes conversion from Text to indexcodes archive 3 disk0/00/00/31/14 2022-10-13 17:06:27 2025-09-18 14:09:40 2022-10-13 17:06:27 data_collection show Robinson Ailie 0000-0002-4291-6696 TRUE 41ac762cdc45f7b568d9e78c431e64fb Versteeg Bart FALSE Shafi Abdurahman Oumer FALSE Clatworthy Innes FALSE Shuka Gemeda FALSE Debe Dereje FALSE Hordofa Gebreyes FALSE Reis de Oliveira Gomes Laura TRUE b7c2f72b749e4447ebdda040ecc4541b Abraham Aga Muluadam FALSE Dumessa Gebeyehu FALSE Sarah Virginia FALSE Macleod David 0000-0002-2371-5709 TRUE c5b5e33838dc4dceb66a6aa3cded6cd7 Last Anna R. TRUE e6a87beb8c490a4cabc8b8cdaf27abab Burton Matthew J. 0000-0003-1872-9169 TRUE 6bffd9753caf0a6a06263d2bdf463bbe Logan James G. 0000-0001-9186-2113 TRUE 6891d5a58f3b75dbc9579858ee082d57 London School of Hygiene & Tropical Medicine, London, United Kingdom Natural History Museum, London, United Kingdom Fred Hollows Foundation, Addis Ababa, Ethiopia EPID ITCR ITDC Musca sorbens Trachoma Chlamydia trachomatis transmission vector behaviour Ethiopia fly-eye contact. A population-based survey of 247 households was conducted in Shashemane district, Oromia Region, Ethiopia, between April and June 2018. Households were positively selected to include at least one child aged 1–9 years, resident on the day of enumeration. These datasets contain entomology from this survey; including fly-eye contact data, fly positivity for Chlamydia trachomatis and other entomological data (fly species, sex). Experiments were also conducted in the London School of Hygiene & Tropical Medicine to investigate transmission of Chlamydia trachomatis by Musca sorbens, those data are reported here. 2022-10-14 completed London School of Hygiene & Tropical Medicine 10.17037/DATA.00003114 Dataset London School of Hygiene & Tropical Medicine, London, United Kingdom Observation.Field.Participant Observation.Laboratory.Nonparticipant 18 JVX vector_control public London, United Kingdom Wellcome Trust Fred Hollows Foundation Stronger SAFE Stronger SAFE Dataset Quantitative Field data: Per household, fly contacts on two children aged 2-9 years were observed for ten minutes. If children were unavailable or there were not two children aged 2-9 years, children aged >9 years were observed (with a preference for the youngest), if no children were present adults were observed. Fly contact observations were made outside in a shady area, and two entomological field workers tallied fly-eye, -nose and -mouth contacts. The face was filmed (using a tripod-mounted Nikon D7200 single-lens reflex camera) for the 10-min observation period. Flies landing on individual’s faces were caught, killed, and transported on ice to our local laboratory as previously described. The observed child sat on a chair and for 15 minutes, or until 10 nets were used, flies landing on the child’s face were disturbed and caught in a sterile net. In the laboratory, flies were identified with keys as M. sorbens, M. domestica, ‘other’ (clearly another species) or ‘unknown’ (unidentifiable) using dissecting microscopes. Musca sorbens and M. domestica were sexed; flies were temporarily stored at -20˚C before transfer to a -80˚C freezer, where they were stored until DNA extraction and testing. Laboratory data: Internal and external recovery of Ct DNA was tested using six female flies (experiment 1), six male flies (experiment 2), and six males/six females (experiment 3). Musca sorbens (broad frons), colonised in 2018, were reared at the London School of Hygiene & Medicine according to previously published methods. Flies of known and uniform age were starved of food (milk/sugar) and water prior to feeding experiments. After starving, surviving flies were put into individual containers covered by a square of mesh with a small hole in the middle plugged with cotton wool. In a microbiological safety cabinet, flies were fed aliquots of Chlamydia trachomatis culture (culture lysate containing EB at approximately 1000 EB/uL) by pipette through the hole in the mesh. All samples were stored immediately in sterile tubes at -20oC. DNA extraction, PCR quantification and load estimation was performed as for the field samples. 7.31038 38.808 7.05627 38.4585 51.5365 -0.0221999 51.4476 -0.2227 en 2018-04 2022-01 https://doi.org/10.1371/journal.pntd.0012280 paper Stronger SAFE Wellcome Trust http://dx.doi.org/10.13039/100010269 Stronger SAFE Fred Hollows Foundation 2022-10-14