217 18 2046 2 7010 document 2046 SupplementaryTableS1.xls application/msword 92cbce48bf227a173af9634704c31ac9076a61c5ac689119aacf9a3853ad41a0 SHA256 68096 2016-06-02 09:38:04 https://datacompass.lshtm.ac.uk/id/eprint/217/1/SupplementaryTableS1.xls 217 1 1 application/msword Dataset en public cc_by

SupplementaryTableS1.xls

data indefinite List of Cytochrome b sequences typed for the present study and those retrieved from GenBank. Strains 1–124 were typed in the present study (including samples 1–34 sampled in this study from Pakistan). Strains 125–165 were retrieved from GenBank 2047 2 7012 document 2047 SupplementaryTableS2.xls application/msword da446a72871f011c2def4393dfda3dbdb9ddfac0d62bde254388bdab3fa78838 SHA256 48128 2016-06-02 09:38:07 https://datacompass.lshtm.ac.uk/id/eprint/217/2/SupplementaryTableS2.xls 217 2 2 application/msword Dataset en public cc_by

SupplementaryTableS2.xls

data indefinite List of internal transcribed spacer 2 sequences typed for the present study and those retrieved from GenBank. Strains 1–10 were typed in the present study (including samples 1–4 sampled in this study). Strains 11–68 were retrieved from GenBank archive 3 disk0/00/00/02/17 2016-06-02 09:39:35 2021-07-09 11:22:03 2016-06-02 09:39:35 data_collection show Khan Nazma Habib FALSE Messenger Louisa A. 0000-0002-3107-6214 FALSE 99b1dfbf56d60edfffbbb030daa7453a Wahid Sobia FALSE 68baed21c3a1610ddefb87261c24b6d2 Sutherland Colin J. 0000-0003-1592-6407 FALSE 002759e7420e57e8650abb2c283b6af5 London School of Hygiene & Tropical Medicine University of Peshawa ITIM ITPM Leishmania Cytochrome b (cytb) Internal transcribed spacer 2 (its2) Khyber Pakhtunkhwa (KP) Several species of the genus Leishmania are causative agents of cutaneous leishmaniasis in Pakistan. This study aimed to determine phylogenetic placement of Leishmania species causing cutaneous leishmaniasis in Khyber Pakhtunkhwa province, Pakistan (34 Leishmaniatropica, 3 Leishmaniainfantum), in-relation to species from other geographical areas using gene sequences encoding cytochrome b (cytb) and internal transcribed spacer 2 (its2). Based on cytochrome b sequence analysis, L. tropica strains from Pakistan and other geographical regions were differentiated into two genotype groups, A and B. Within the province, five distinct L. tropica genotypes were recognized; two in group A, three in group B. Two L. infantum isolates from the province were closely associated with both Afro-Eurasian and American species of the Leishmaniadonovani complex, including Leishmaniachagasi, L. infantum and L. donovani from Sudan and Ethiopia; while a third L. infantum isolate could not be differentiated from visceralizing Kenyan and Indian L. donovani. We observed apposite phylogenetic placement of CL-causing L. tropica and L. infantum from Khyber Pakhtunkhwa. Affinities ascribed to Leishmania spp. From the region are valuable in tracing potential importation of leishmaniasis. 2016-05-24 published Experimental Parasitology 10.1016/j.exppara.2016.05.006 Dataset London School of Hygiene & Tropical Medicine University of Peshawa Experiment.FieldIntervention public Dataset Quantitative 34.1485 71.8327 33.8297 71.2724 en http://researchonline.lshtm.ac.uk/id/eprint/2550087 2016-05-24