10.17037/DATA.00004261
Pyrethroid-chlorfenapyr nets have been recommended for malaria control by the World Health Organisation (WHO) after an alpha-cypermethrin-chlorfenapyr net showed improved impact in epidemiological trials. PermaNet® Dual is a new deltamethrin-chlorfenapyr net developed by Vestergaard Sàrl to expand options to control programmes. A series of laboratory studies were performed according to WHO guidelines to assess the regeneration time, efficacy and wash-resistance of PermaNet® Dual. Regeneration time was determined by subjecting net pieces to cone bioassays and tunnel tests before and 0, 1, 2, 3, 5 and 7 days after washing. The wash-resistance of PermaNet® Dual was evaluated compared to WHO-prequalified pyrethroid-only (PermaNet® 2.0) and pyrethroid-chlorfenapyr (Interceptor® G2) nets by testing net pieces washed 0, 1, 3, 5, 10, 15 and 20 times in cone bioassays and tunnel tests. Tests were performed with susceptible and pyrethroid-resistant strains of Anopheles gambiae to assess the pyrethroid and chlorfenapyr components separately. Net pieces were also analysed to determine insecticide content. In regeneration time studies, the biological activity of the deltamethrin and chlorfenapyr components of PermaNet® Dual regenerated within one day after washing and a 1-day washing interval was adopted for wash-resistance studies. PermaNet® Dual induced high mortality (98%) and blood-feeding inhibition (98%) of the susceptible strain after 20 washes fulfilling WHO efficacy criteria in tunnel tests (≥80% mortality, ≥90% blood-feeding inhibition). Similar results were obtained with PermaNet® 2.0 (99% mortality, 99% blood-feeding inhibition) and Interceptor® G2 (99% mortality, 98% blood-feeding inhibition) washed 20 times. In wash-resistance tunnel tests against the pyrethroid-resistant strain, PermaNet® Dual washed 20 times induced high mortality (91%) and blood-feeding inhibition (73%), which was similar to Interceptor® G2 (87% mortality, 79% blood-feeding inhibition) and superior to PermaNet® 2.0 (47% mortality, 68% blood-feeding inhibition). PermaNet® Dual fulfilled WHO efficacy criteria in laboratory bioassays and showed potential to improve control of pyrethroid-resistant malaria vectors.
A series of laboratory studies were performed according to WHO guidelines to assess the regeneration time, efficacy and wash-resistance of PermaNet® Dual. Regeneration time was determined by subjecting net pieces to cone bioassays and tunnel tests before and 0, 1, 2, 3, 5 and 7 days after washing. The wash-resistance of PermaNet® Dual was evaluated compared to WHO-prequalified pyrethroid-only (PermaNet® 2.0) and pyrethroid-chlorfenapyr (Interceptor® G2) nets by testing net pieces washed 0, 1, 3, 5, 10, 15 and 20 times in cone bioassays and tunnel tests. Tests were performed with susceptible and pyrethroid-resistant strains of Anopheles gambiae to assess the pyrethroid and chlorfenapyr components separately. Net pieces were also analysed to determine insecticide content.
Tests were performed with susceptible and pyrethroid-resistant strains of Anopheles gambiae.
| Organisation | Ethics ID | Other information |
| London School of Hygiene & Tropical Medicine | 26429 |
Anopheles gambiae, Vector Control, Insecticide-treated net
English
| Project name | Funder/sponsor | Grant number |
| New nets project | UNITAID |
| Forename | Surname | Faculty / Dept | Institution | Role |
| Thomas | Syme | Faculty of Infectious and Tropical Diseases / Dept of Disease Control | London School of Hygiene & Tropical Medicine | Co-Investigator |
| Boris | N'dombidje | The CREC/LSHTM Collaborative Research Programme | Data Collector | |
| Damien | Todjinou | The CREC/LSHTM Collaborative Research Programme | Data Collector | |
| Victoria | Ariori | The CREC/LSHTM Collaborative Research Programme | Data Collector | |
| Corine | Ngufor | Faculty of Infectious and Tropical Diseases / Dept of Disease Control | London School of Hygiene & Tropical Medicine | Principal Investigator |
The CREC/LSHTM Collaborative Research Programme is a research partnership set up in 2003, between the London School of Hygiene & Tropical Medicine in the UK and the Centre de Recherche Entomologique de Cotonou held at the Ministry of Health in Benin.
| Filename | Description | Access status | Licence |
| Lab_evaluation_of_PermDual_data | Laboratory evaluation of PermaNet Dual raw data (Excel format) | Open | Creative Commons Attribution (CC BY) |
| Cone_bioassays_data | Cone bioassays data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| Tunnel_tests_data | Tunnel tests data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| Lab_evaluation_of_PermDual_data_codebook | Codebook for Cone bioassays and tunnel tests data tables | Open | Creative Commons Attribution (CC BY) |
| Lab_evaluation_of_PermDual_summary | Summary statistics for laboratory evaluation of PermaNet Dual data (Excel format) | Open | Creative Commons Attribution (CC BY) |
| Chemical_analysis | Chemical analysis data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| RT_Cones | RT Cones data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| RT_Tunnels | RT Tunnels data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| WR_Cones | WR Cones data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |
| WR_Tunnels | WR Tunnels data table (tab-delimited) | Open | Creative Commons Attribution (CC BY) |