Search strategies for: Diagnostic accuracy of tests to detect Hepatitis B surface antigen: a meta-analysis and review of the literature Final report, December 2016. 1. Literature search methodology The literature search strategies were developed by a medical librarian with expertise in systematic review searching, with input from RP and JT. The search included synonyms and subject headings on three topics, thus three search strategies were devised. The first search looked at three search concepts: hepatitis B, diagnostic tests and diagnostic accuracy. The second search looked at three search concepts: hepatitis B, screening and testing strategies. The third search looked at three search concepts: hepatitis B, RNA tests and diagnosic accuracy. The following databases were searched: MEDLINE, EMBASE, the Cochrane Central Register of Controlled Trials, Science Citation Index Expanded, Conference Proceedings Citation Index-Science, SCOPUS, Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) and WHO Global Index Medicus. Searches were conduced between during April 2015. The search was supplemented by searching for ongoing studies in WHO’s International Clinical Trials Registry. The search aimed to retrieve all relevant studies regardless of publication status, however, where possible, the search strategy was limited to the English language and human subjects. 2. Search strategies Search 1 OvidSP Medline Searched on 20 April 2015 from 1946 – April week 2 2015. 1 Hepatitis, Viral, Human/ (10382) 2 Hepatitis Viruses/ (1363) 3 Hepatitis Antibodies/ (5082) 4 exp Hepadnaviridae Infections/ (47484) 5 Hepatitis B Antibodies/ (8638) 6 Hepatitis B virus/ (20604) 7 Hepadnaviridae/ (192) 8 Hepatitis B Surface Antigens/ (17007) 9 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (64488) 10 hbsag.ti,ab. (15146) 11 or/1-10 [HEPATITIS B] (87943) 12 exp Reagent Kits, Diagnostic/ (17747) 13 ((rapid or point of care or near patient or poc or poct or bedside) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (63080) 14 (radt or radts or rdt or rdts).ti,ab. (909) 15 rapid test*.ti,ab. (3400) 16 exp Enzyme-Linked Immunosorbent Assay/ (127391) 17 Immunoassay/ (23237) 18 Immunoenzyme Techniques/ (64864) 19 (enzyme-linked immunosorbent assay or ELISA).ti,ab. (139374) 20 (enzyme adj2 (immunoassay* or immuno-assay* or immunosorbent)).ti,ab. (83849) 21 ((antigen* or antibod*) adj3 detect*).ti,ab. (59427) 22 or/12-21 [RAPID DIAGNOSTIC TESTS] (394724) 23 exp "Sensitivity and Specificity"/ (435087) 24 (diagnos* accura* or sensitiv* or specific* or valid*).ti,ab. (3016884) 25 roc curve.ti,ab. (10226) 26 positive predictive value.ti,ab. (25496) 27 negative predictive value.ti,ab. (20415) 28 or/23-27 [DIAGNOSTIC ACCURACY] (3235789) 29 11 and 22 and 28 (3103) 30 Humans/ (13846846) 31 Animals/ (5442465) 32 30 and 31 (1513142) 33 31 not 32 [ALL ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (3929323) 34 29 not 33 (2856) 35 limit 34 to english language (2345) OvidSP Embase Searched on 20 April 2015 from 1947 – 2015 April 17. 1 hepatitis virus/ (4410) 2 hepatitis antibody/ (2216) 3 exp hepadnaviridae/ (42214) 4 hepatitis B surface antigen/ (27312) 5 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (94627) 6 hbsag.ti,ab. (22290) 7 or/1-6 [HEPATITIS B] (111321) 8 exp diagnostic kit/ (13384) 9 "point of care testing"/ (5530) 10 ((rapid or point of care or near patient or poc or poct or bedside) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (88003) 11 (radt or radts or rdt or rdts).ti,ab. (1652) 12 rapid test*.ti,ab. (5049) 13 enzyme linked immunosorbent assay/ (229634) 14 immunoassay/ (48491) 15 enzyme immunoassay/ (36845) 16 enzyme linked immunospot assay/ (6789) 17 enzyme multiplied immunoassay technique/ (768) 18 (enzyme-linked immunosorbent assay or ELISA).ti,ab. (204052) 19 (enzyme adj2 (immunoassay* or immuno-assay* or immunosorbent)).ti,ab. (98704) 20 antigen detection/ (18155) 21 antibody detection/ (34389) 22 ((antigen* or antibod*) adj3 detect*).ti,ab. (76053) 23 or/8-22 [RAPID DIAGNOSTIC TESTS] (525203) 24 "sensitivity and specificity"/ (221828) 25 diagnostic accuracy/ (189329) 26 (diagnos* accura* or sensitiv* or specific* or valid*).ti,ab. (4113104) 27 roc curve.ti,ab. (20232) 28 positive predictive value.ti,ab. (37541) 29 negative predictive value.ti,ab. (31612) 30 or/24-29 [DIAGNOSTIC ACCURACY] (4280338) 31 7 and 23 and 30 (4018) 32 human/ (15785497) 33 animal/ (1646303) 34 32 and 33 (404532) 35 33 not 34 [ALL ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (1241771) 36 31 not 35 (3963) 37 limit 36 to english language (3344) Web of Science Search was conducted on the Science Citation Index Expanded (1970 - 20 April 2015) and the Conference Proceedings Citation Index-Science (1990 - 20 April 2015) 1 TOPIC: ("hepatitis-b" OR "hep-b" OR (hepatitis near/5 b) OR (hep near/5 b) OR hbv) (79,505) 2 TOPIC: (hbsag) (12,160) 3 #2 OR #1 (81,526) 4 TOPIC: ((rapid near/5 test) or (rapid near/5 tests) or (rapid near/5 testing) or (rapid near/5 detect*) or (rapid near/5 diagnos*) or (rapid near/5 screen*) or (rapid near/5 kit) or (rapid near/5 kits) or (rapid near/5 assay*) or (rapid near/5 device*)) (77,863) 5 TOPIC: (("point of care" near/5 test) or ("point of care" near/5 tests) or ("point of care" near/5 testing) or ("point of care" near/5 detect*) or ("point of care" near/5 diagnos*) or ("point of care" near/5 screen*) or ("point of care" near/5 kit) or ("point of care" near/5 kits) or ("point of care" near/5 assay*) or ("point of care" near/5 device*)) (5,974) 6 TOPIC: (("near patient" near/5 test) or ("near patient" near/5 tests) or ("near patient" near/5 testing) or ("near patient" near/5 detect*) or ("near patient" near/5 diagnos*) or ("near patient" near/5 screen*) or ("near patient" near/5 kit) or ("near patient" near/5 kits) or ("near patient" near/5 assay*) or ("near patient" near/5 device*)) (423) 7 TOPIC: ((poc near/5 test) or (poc near/5 tests) or (poc near/5 testing) or (poc near/5 detect*) or (poc near/5 diagnos*) or (poc near/5 screen*) or (poc near/5 kit) or (poc near/5 kits) or (poc near/5 assay*) or (poc near/5 device*)) (866) 8 TOPIC: ((poct near/5 test) or (poct near/5 tests) or (poct near/5 testing) or (poct near/5 detect*) or (poct near/5 diagnos*) or (poct near/5 screen*) or (poct near/5 kit) or (poct near/5 kits) or (poct near/5 assay*) or (poct near/5 device*)) (522) 9 TOPIC: ((bedside near/5 test) or (bedside near/5 tests) or (bedside near/5 testing) or (bedside near/5 detect*) or (bedside near/5 diagnos*) or (bedside near/5 screen*) or (bedside near/5 kit) or (bedside near/5 kits) or (bedside near/5 assay*) or (bedside near/5 device*)) (2,705) 10 TOPIC: (radt or radts or rdt or rdts) (1,406) 11 TOPIC: ("rapid test*") (3,783) 12 TOPIC: ("enzyme-linked immunosorbent assay" or ELISA) (141,435) 13 TOPIC: ((enzyme near/2 immunoassay*) or (enzyme near/2 immuno-assay*) or (enzyme near/2 immunosorbent)) (85,660) 14 TOPIC: ((antigen* near/3 detect*) or (antibod* near/3 detect*)) (56,976) 15 #14 OR #13 OR #12 OR #11 OR #10 OR #9 OR #8 OR #7 OR #6 OR #5 OR #4 (286,936) 16 TOPIC: ("diagnos* accura*" or sensitiv* or specific* or valid*) (4,557,124) 17 TOPIC: ("roc curve") (12,767) 18 TOPIC: ("positive predictive value") (23,706) 19 TOPIC: ("negative predictive value") (18,947) 20 #19 OR #18 OR #17 OR #16 (4,566,667) 21 #20 AND #15 AND #3 (1,789) 22 #20 AND #15 AND #3 Refined by: LANGUAGES: ( ENGLISH ) (1,720) Scopus Search was conducted on 20 April 2015 TITLE-ABS-KEY (("heptatitis-b" OR "hep-b" OR (hepatitis W/5 b) OR (hep W/5 b) OR hbv OR hbsag) AND (((rapid OR "point of care" OR "near patient" OR poc OR poct OR bedside) W/5 (tests OR test OR testing OR detect* OR diagnos* OR screen* OR kit OR kits OR assay* OR device*)) OR radt OR radts OR rdt OR rdts OR "rapid test*" OR "enzyme-linked immunosorbent assay" OR elisa OR (enzyme W/2 (immunoassay* OR immuno-assay* OR immunosorbent)) OR ((antibod* OR anigen*) W/3 detect*)) AND ("diagnos* accura*" OR sensitiv* OR specific* OR valid* OR "roc curve" OR "positive predictive value" OR "negative predictive value")) AND (LIMIT-TO (LANGUAGE, "English")) (3,605) Cochrane Central Register of Controlled Trials, Wiley The search was run on 20 April 2015 1 MeSH descriptor: [Hepatitis, Viral, Human] this term only 2 MeSH descriptor: [Hepatitis Viruses] this term only 3 MeSH descriptor: [Hepatitis Antibodies] this term only 4 MeSH descriptor: [Hepadnaviridae Infections] explode all trees 5 MeSH descriptor: [Hepatitis B Antibodies] this term only 6 MeSH descriptor: [Hepatitis B virus] this term only 7 MeSH descriptor: [Hepadnaviridae] this term only 8 MeSH descriptor: [Hepatitis B Surface Antigens] explode all trees 9 "hepatitis-b":ti,ab,kw (Word variations have been searched) 10 "hep-b":ti,ab,kw (Word variations have been searched) 11 hepatitis near/5 b:ti,ab,kw (Word variations have been searched) 12 hep near/5 b:ti,ab,kw (Word variations have been searched) 13 hbv:ti,ab,kw (Word variations have been searched) 14 hbsag:ti,ab,kw (Word variations have been searched) 15 #1 or #2 or #3 or #4 or #5 or #6 or #7 or #8 or #9 or #10 or #11 or #12 or #13 or #14 16 MeSH descriptor: [Reagent Kits, Diagnostic] explode all trees 17 (rapid or "point of care" or "near patient" or poc or poct or bedside) near/5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*):ti,ab,kw (Word variations have been searched) 18 radt or radts or rdt or rdts:ti,ab,kw (Word variations have been searched) 19 "rapid test*":ti,ab,kw (Word variations have been searched) 20 MeSH descriptor: [Enzyme-Linked Immunosorbent Assay] explode all trees 21 enzyme near/2 (immunoassay* or immuno-assay* or immunosorbent):ti,ab,kw (Word variations have been searched) 22 (antigen* or antibod*) near/3 detect*:ti,ab,kw (Word variations have been searched) 23 MeSH descriptor: [Immunoassay] this term only 24 MeSH descriptor: [Immunoenzyme Techniques] this term only 25 "enzyme-linked immunosorbent assay" or ELISA:ti,ab,kw (Word variations have been searched) 26 #16 or #17 or #18 or #19 or #20 or #21 or #22 or #23 or #24 or #25 27 MeSH descriptor: [Sensitivity and Specificity] explode all trees 28 diagnos* accura* or sensitiv* or specific* or valid*:ti,ab,kw (Word variations have been searched) 29 "roc curve":ti,ab,kw (Word variations have been searched) 30 "positive predictive value":ti,ab,kw (Word variations have been searched) 31 "negative predictive value":ti,ab,kw (Word variations have been searched) 32 #27 or #28 or #29 or #30 or #31 33 #15 and #26 and #32 The search found 64 trials. Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) (BIREME interface) LILACS was searched on 20 April 2015 ("hepatitis b" or "hep b" or "hbv" or "hbsag") and ("rapid test$" or "point of care test$" or "near patient test$" or "poc test$" or poct or "bedside test$" or "rapid detect$" or "point of care detect$" or "near patient detect$" or "poc detect$" or "bedside detect$" or "rapid diagnos$" or "point of care diagnos$" or "near patient diagnos$" or "poc diagnos$" or "bedside diagnos$" or "rapid screen$" or "point of care screen$" or "near patient screen$" or "poc screen$" or "bedside screen$" or "rapid kit$" or "point of care kit$" or "near patient kit$" or "poc kit$" or "bedside kit$" or "rapid assay$" or "point of care assay$" or "near patient assay$" or "poc assay$" or "bedside assay$" or "rapid device$" or "point of care device$" or "near patient device$" or "poc device$" or "bedside device$" or radt or radts or rdt or rdts or "enzyme-linked immunosorbent assay" or "antigen$ detect$" or "antibod$ detect$" or elisa or immunoassay or immunoenzyme or "immuno-assay") and ("diagnos$ accura$" or sensitiv$ or specific$ or valid$ or "roc curve" or "positive predictive value" or "negative predictive value") (33) WHO Global Index Medicus The database was searched on 22 April 2015 SUBJECT: (("Hepatitis, Viral, Human" OR "Hepatitis Viruses" OR "Hepatitis B virus" OR "Hepatitis Antibodies" OR "Hepadnaviridae Infections" OR "Hepatitis B Antibodies" OR "Hepatitis B Virus" OR "Hepadnaviridae" OR "Hepatitis B Surface Antigens") AND ("Reagent Kits, Diagnostic" OR "Enzyme-Linked Immunosorbent Assay" OR "Immunoassay" OR "Immunoenzyme Techniques") AND ("Sensitivity and Specificity")) (478) Search 2 OvidSP Medline Searched on 27 April 2015 from 1946 – April week 3 2015. 1 Hepatitis, Viral, Human/ (10388) 2 Hepatitis Viruses/ (1363) 3 Hepatitis Antibodies/ (5087) 4 exp Hepadnaviridae Infections/ (47598) 5 Hepatitis B Antibodies/ (8643) 6 Hepatitis B virus/ (20682) 7 Hepadnaviridae/ (192) 8 Hepatitis B Surface Antigens/ (17042) 9 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (64630) 10 hbsag.ti,ab. (15183) 11 or/1-10 [HEPATITIS B] (88104) 12 exp Mass Screening/ (103115) 13 screen*.ti,ab. (451417) 14 12 or 13 [MASS SCREENING] (486787) 15 (one-test* or two-test*).ti,ab. (10516) 16 ("1-test*" or "2-test*").ti,ab. (12661) 17 ((one or two or "1" or "2" or strateg* or algorithm* or approach or procedure* or system*) adj5 (test or tests or 1. testing or detect* or diagnos* or kit or kits or assay* or device*)).ti,ab. (637709) 18 or/15-17 [TESTING ALGORITHM] (640339) 19 11 and 14 and 18 (793) 20 Human/ (13864285) 21 Animal/ (5446931) 22 20 and 21 (1515064) 23 21 not 22 [ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (3931867) 24 19 not 23 (787) 25 limit 24 to english language (663) OvidSP Embase Searched on 20 April 2015 from 1947 – 2015 week 17 1 hepatitis virus/ (4413) 2 hepatitis antibody/ (2219) 3 exp hepadnaviridae/ (42296) 4 hepatitis B surface antigen/ (27371) 5 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (94816) 6 hbsag.ti,ab. (22336) 7 or/1-6 [HEPATITIS B] (111529) 8 exp screening/ (507728) 9 screen*.ti,ab. (666419) 10 8 or 9 [MASS SCREENING] (886147) 11 (one-test* or two-test*).ti,ab. (14770) 12 ("1-test*" or "2-test*").ti,ab. (10488) 13 ((one or two or "1" or "2" or strateg* or algorithm* or approach or procedure* or system*) adj5 (test or tests or 1 testing or detect* or diagnos* or kit or kits or assay* or device*)).ti,ab. (942900) 14 or/11-13 [TESTING ALGORITHM] (946413) 15 7 and 10 and 14 (1668) 16 human/ (15822451) 17 animal/ (1650963) 18 16 and 17 (406393) 19 17 not 18 [ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (1244570) 20 15 not 19 (1664) 21 limit 20 to english language (1498) Web of Science Search was conducted on the Science Citation Index Expanded (1970 - 27 April 2015) and the Conference Proceedings Citation Index-Science (1990 - 27 April 2015) 1 TOPIC: ("hepatitis-b" OR "hep-b" OR (hepatitis near/5 b) OR (hep near/5 b) OR hbv) (79,598) 2 TOPIC: (hbsag) (12,172) 3 #2 OR #1 (81,619) 4 TOPIC: (screen*) (564,341) 5 TOPIC: ("one-test*" or "two-test*" or "1-test*" or "2-test*") (33,693) 6 TOPIC: ((one near/5 test) or (two near/5 test) or ("1" near/5 test) or ("2" near/5 test) or (strateg* near/5 test) or (algorithm* near/5 test) or (approach near/5 test) or (procedure* near/5 test) or (system* near/5 test)) (580,855) 7 TOPIC: ((one near/5 tests) or (two near/5 tests) or ("1" near/5 tests) or ("2" near/5 tests) or (strateg* near/5 tests) or (algorithm* near/5 tests) or (approach near/5 tests) or (procedure* near/5 tests) or (system* near/5 tests)) (580,855) 8 TOPIC: ((one near/5 testing) or (two near/5 testing) or ("1" near/5 testing) or ("2" near/5 testing) or (strateg* near/5 testing) or (algorithm* near/5 testing) or (approach near/5 testing) or (procedure* near/5 testing) or (system* near/5 testing)) (580,855) 9 TOPIC: ((one near/5 detect*) or (two near/5 detect*) or ("1" near/5 detect*) or ("2" near/5 detect*) or (strateg* near/5 detect*) or (algorithm* near/5 detect*) or (approach near/5 detect*) or (procedure* near/5 detect*) or (system* near/5 detect*)) (488,294) 10 TOPIC: ((one near/5 diagnos*) or (two near/5 diagnos*) or ("1" near/5 diagnos*) or ("2" near/5 diagnos*) or (strateg* near/5 diagnos*) or (algorithm* near/5 diagnos*) or (approach near/5 diagnos*) or (procedure* near/5 diagnos*) or (system* near/5 diagnos*)) (223,463) 11 TOPIC: ((one near/5 kit) or (two near/5 kit) or ("1" near/5 kit) or ("2" near/5 kit) or (strateg* near/5 kit) or (algorithm* near/5 kit) or (approach near/5 kit) or (procedure* near/5 kit) or (system* near/5 kit)) (8,867) 12 TOPIC: ((one near/5 kits) or (two near/5 kits) or ("1" near/5 kits) or ("2" near/5 kits) or (strateg* near/5 kits) or (algorithm* near/5 kits) or (approach near/5 kits) or (procedure* near/5 kits) or (system* near/5 kits)) (8,867) 13 TOPIC: ((one near/5 assay*) or (two near/5 assay*) or ("1" near/5 assay*) or ("2" near/5 assay*) or (strateg* near/5 assay*) or (algorithm* near/5 assay*) or (approach near/5 assay*) or (procedure* near/5 assay*) or (system* near/5 assay*)) (115,506) 14 TOPIC: ((one near/5 device*) or (two near/5 device*) or ("1" near/5 device*) or ("2" near/5 device*) or (strateg* near/5 device*) or (algorithm* near/5 device*) or (approach near/5 device*) or (procedure* near/5 device*) or (system* near/5 device*)) (119,146) 15 #14 OR #13 OR #12 OR #11 OR #10 OR #9 OR #8 OR #7 OR #6 OR #5 (1,440,670) 16 #15 AND #4 AND #3 (1,004) Scopus Search was conducted on 27 April 2015 TITLE-ABS-KEY (("heptatitis-b" OR "hep-b" OR (hepatitis W/5 b) OR (hep W/5 b) OR hbv OR hbsag) AND (screen*) AND (((one or two or "1" or "2" or strateg* or algorithm* or approach or procedure* or system*) W/5 (test or tests or testing or detect* or diagnos* or kit or kits or assay* or device*)) OR "one-test*" OR "two-test*" OR "1-test*" OR "2-test*")) AND (LIMIT-TO (LANGUAGE, "English")) (1,600) Cochrane Central Register of Controlled Trials, Wiley The search was run on 27 April 2015 1 MeSH descriptor: [Hepatitis, Viral, Human] this term only 2 MeSH descriptor: [Hepatitis Viruses] this term only 3 MeSH descriptor: [Hepatitis Antibodies] this term only 4 MeSH descriptor: [Hepadnaviridae Infections] explode all trees 5 MeSH descriptor: [Hepatitis B Antibodies] this term only 6 MeSH descriptor: [Hepatitis B virus] this term only 7 MeSH descriptor: [Hepadnaviridae] this term only 8 MeSH descriptor: [Hepatitis B Surface Antigens] explode all trees 9 "hepatitis-b":ti,ab,kw (Word variations have been searched) 10 "hep-b":ti,ab,kw (Word variations have been searched) 11 hepatitis near/5 b:ti,ab,kw (Word variations have been searched) 12 hep near/5 b:ti,ab,kw (Word variations have been searched) 13 hbv:ti,ab,kw (Word variations have been searched) 14 hbsag:ti,ab,kw (Word variations have been searched) 15 #1 or #2 or #3 or #4 or #5 or #6 or #7 or #8 or #9 or #10 or #11 or #12 or #13 or #14 16 MeSH descriptor: [Mass Screening] explode all trees 17 screen*:ti,ab,kw (Word variations have been searched) 18 #16 or #17 19 "one-test" or "two-test" or "1-test" or "2-test":ti,ab,kw (Word variations have been searched) 20 (one or two or "1" or "2" or strateg* or algorithm* or approach or procedure* or system*) near/5 (test or tests or testing or detect* or diagnos* or kit or kits or assay* or device*):ti,ab,kw (Word variations have been searched) 21 #19 or #20 22 #15 and #18 and #21 The search found 30 trials. Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) (BIREME interface) LILACS was searched on 27 April 2015 ("hepatitis b" or "hep b" or "hbv" or "hbsag") and (screen$) (394) WHO Global Index Medicus The database was searched on 27 April 2015 SUBJECT: (("Hepatitis, Viral, Human" OR "Hepatitis Viruses" OR "Hepatitis B virus" OR "Hepatitis Antibodies" OR "Hepadnaviridae Infections" OR "Hepatitis B Antibodies" OR "Hepatitis B Virus" OR "Hepadnaviridae" OR "Hepatitis B Surface Antigens") AND ("mass screening")) (826) Search 3 OvidSP Medline Searched on 30 April 2015 from 1946 – April week 4 2015. 1 Hepatitis, Viral, Human/ (10392) 2 Hepatitis Viruses/ (1363) 3 Hepatitis Antibodies/ (5089) 4 exp Hepadnaviridae Infections/ (47642) 5 Hepatitis B Antibodies/ (8649) 6 Hepatitis B virus/ (20709) 7 Hepadnaviridae/ (192) 8 Hepatitis B Surface Antigens/ (17051) 9 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (64690) 10 hbsag.ti,ab. (15196) 11 or/1-10 [HEPATITIS B] (88174) 12 exp Nucleic Acid Amplification Techniques/ (386992) 13 ((nucleic acid or nucleic-acid amplification) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (5101) 14 (NAT or NAAT).ti,ab. (4731) 15 ((reverse transcriptase or reverse transcription or real time) adj1 (PCR or polymerase chain reaction)).ti,ab. (115563) 16 (RT-PCR or RT-qPCR or PCR or qPCR).ti,ab. (334985) 17 ((quantification or quantify or quantitative) adj1 (DNA or RNA)).ti,ab. (1950) 18 ((qualitative or descriptive) adj1 (DNA or RNA)).ti,ab. (102) 19 ((rna or dna) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (100420) 20 or/12-19 [NAT] (634329) 21 exp "Sensitivity and Specificity"/ (436384) 22 (diagnos* accura* or sensitiv* or specific* or valid*).ti,ab. (3024410) 23 roc curve.ti,ab. (10282) 24 positive predictive value.ti,ab. (25606) 25 negative predictive value.ti,ab. (20505) 26 or/21-25 [DIAGNOSTIC ACCURACY] (3243954) 27 11 and 20 and 26 (3131) 28 Humans/ (13876833) 29 Animals/ (5450338) 30 28 and 29 (1516455) 31 29 not 30 [ALL ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (3933883) 32 27 not 31 (2954) 33 limit 32 to english language (2566) OvidSP Embase Searched on 30 April 2015 from 1947 – 2015 week 17 1 hepatitis virus/ (4413) 2 hepatitis antibody/ (2219) 3 exp hepadnaviridae/ (42296) 4 hepatitis B surface antigen/ (27371) 5 (heptatitis-b or hep-b or (hepatitis adj5 b) or (hep adj5 b) or hbv).ti,ab. (94816) 6 hbsag.ti,ab. (22336) 7 or/1-6 [HEPATITIS B] (111529) 8 "sensitivity and specificity"/ (222664) 9 diagnostic accuracy/ (189508) 10 (diagnos* accura* or sensitiv* or specific* or valid*).ti,ab. (4122539) 11 roc curve.ti,ab. (20317) 12 positive predictive value.ti,ab. (37648) 13 negative predictive value.ti,ab. (31708) 14 or/8-13 [DIAGNOSTIC ACCURACY] (4290175) 15 exp nucleic acid analysis/ (745409) 16 ((nucleic acid or nucleic-acid amplification) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (7337) 17 (NAT or NAAT).ti,ab. (8086) 18 ((reverse transcriptase or reverse transcription or real time) adj1 (PCR or polymerase chain reaction)).ti,ab. (155657) 19 (RT-PCR or RT-qPCR or PCR or qPCR).ti,ab. (498163) 20 ((quantification or quantify or quantitative) adj1 (DNA or RNA)).ti,ab. (2727) 21 ((qualitative or descriptive) adj1 (DNA or RNA)).ti,ab. (146) 22 ((rna or dna) adj5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*)).ti,ab. (128519) 23 or/15-22 [NAT] (988748) 24 7 and 14 and 23 (4894) 25 human/ (15822451) 26 animal/ (1650963) 27 25 and 26 (406393) 28 26 not 27 [ANIMAL STUDIES WHICH DO NOT INCLUDE COMPARISON WITH HUMANS] (1244570) 29 24 not 28 (4852) 30 limit 29 to english language (4300) Web of Science Search was conducted on the Science Citation Index Expanded (1970 – 30 April 2015) and the Conference Proceedings Citation Index-Science (1990 – 30 April 2015) 1 TOPIC: ("hepatitis-b" OR "hep-b" OR (hepatitis near/5 b) OR (hep near/5 b) OR hbv) (79,713) 2 TOPIC: (hbsag) (12,201) 3 #1 or #2 (81,735) 4 TOPIC: (("nucleic-acid amplification" near/5 test) or ("nucleic-acid amplification" near/5 tests) or ("nucleic-acid amplification" near/5 testing) or ("nucleic-acid amplification" near/5 detect*) or ("nucleic-acid amplification" near/5 diagnos*) or ("nucleic-acid amplification" near/5 screen*) or ("nucleic-acid amplification" near/5 kit) or ("nucleic-acid amplification" near/5 kits) or ("nucleic-acid amplification" near/5 assay*) or ("nucleic-acid amplification" near/5 device*) or ("nucleic acid" near/5 test) or ("nucleic acid" near/5 tests) or ("nucleic acid" near/5 testing) or ("nucleic acid" near/5 detect*) or ("nucleic acid" near/5 diagnos*) or ("nucleic acid" near/5 screen*) or ("nucleic acid" near/5 kit) or ("nucleic acid" near/5 kits) or ("nucleic acid" near/5 assay*) or ("nucleic acid" near/5 device*)) (6,933) 5 TOPIC: (NAT or NAAT) (9,080) 6 TOPIC: (("reverse transcriptase" near/2 PCR) or ("reverse transcritase" near/2 "polymerase chain reaction") or ("reverse transcription" near/2 PCR) or ("reverse transcription" near/2 "polymerase chain reaction") or ("real time" near/2 PCR) or ("real time" near/2 "polymerase chain reaction")) (141,467) 7 TOPIC: (RT-PCR or RT-qPCR or PCR or qPCR) (405,526) 8 TOPIC: ((quantification near/2 dna) or (quantify near/2 dna) or (quantitative near/2 dna) or (quantification near/2 rna) or (quantify near/2 rna) or (quantitative near/2 rna) or (qualitative near/2 dna) or (descriptive near/2 dna) or (qualitative near/2 rna) or (descriptive near/2 rna)) (9,935) 9 ((rna near/5 test) or (rna near/5 tests) or (rna near/5 testing) or (rna near/5 detect*) or (rna near/5 diagnos*) or (rna near/5 screen*) or (rna near/5 kit) or (rna near/5 kits) or (rna near/5 assay*) or (rna near/5 device*) or (dna near/5 test) or (dna near/5 tests) or (dna near/5 testing) or (dna near/5 detect*) or (dna near/5 diagnos*) or (dna near/5 screen*) or (dna near/5 kit) or (dna near/5 kits) or (dna near/5 assay*) or (dna near/5 device*)) (137,777) 10 #4 or #5 or #6 or #7 or #8 or #9 (540,983) 11 TOPIC: ("diagnos* accura*" or sensitiv* or specific* or valid*) (4,574,544) 12 TOPIC: ("roc curve") (12,877) 13 TOPIC: ("positive predictive value") (23,803) 14 TOPIC: ("negative predictive value") (19,035) 15 #11 or #12 or #13 or #14 (4,584,137) 16 #3 and #10 and #15 (2,751) 17 #3 and #10 and #15 Refined by: LANGUAGES: (ENGLISH) (2,685) Scopus Search was conducted on 30 April 2015 TITLE-ABS-KEY (("heptatitis-b" OR "hep-b" OR (hepatitis W/5 b) OR (hep W/5 b) OR hbv OR hbsag) AND ((("nucleic acid" OR "nucleic-acid amplification") W/5 (test OR tests OR testing OR detect* OR diagnos* OR screen* OR kit OR kits OR assay* OR device*)) OR nat OR naat OR (("reverse transcriptase" OR "reverse transcription" OR "real time") W/1 (pcr OR "polymerase chain reaction")) OR rt-pcr OR rt-qpcr OR pcr OR qpcr OR ((quantification OR quantify OR quantitative) W/1 (dna OR rna)) OR ((qualitative OR descriptive) W/1 (dna OR rna)) OR ((rna OR dna) W/5 (test OR tests OR testing OR detect* OR diagnos* OR screen* OR kit OR kits OR assay* OR device*))) AND ("diagnos* accura*" OR sensitiv* OR specific* OR valid* OR "roc curve" OR "positive predictive value" OR "negative predictive value")) AND (LIMIT-TO (LANGUAGE, "English")) (3,607) Cochrane Central Register of Controlled Trials, Wiley The search was run on 30 April 2015 1 MeSH descriptor: [Hepatitis, Viral, Human] this term only 2 MeSH descriptor: [Hepatitis Viruses] this term only 3 MeSH descriptor: [Hepatitis Antibodies] this term only 4 MeSH descriptor: [Hepadnaviridae Infections] explode all trees 5 MeSH descriptor: [Hepatitis B Antibodies] this term only 6 MeSH descriptor: [Hepatitis B virus] this term only 7 MeSH descriptor: [Hepadnaviridae] this term only 8 MeSH descriptor: [Hepatitis B Surface Antigens] explode all trees 9 "hepatitis-b":ti,ab,kw (Word variations have been searched) 10 "hep-b":ti,ab,kw (Word variations have been searched) 11 hepatitis near/5 b:ti,ab,kw (Word variations have been searched) 12 hep near/5 b:ti,ab,kw (Word variations have been searched) 13 hbv:ti,ab,kw (Word variations have been searched) 14 hbsag:ti,ab,kw (Word variations have been searched) 15 #1 or #2 or #3 or #4 or #5 or #6 or #7 or #8 or #9 or #10 or #11 or #12 or #13 or #14 16 MeSH descriptor: [Nucleic Acid Amplification Techniques] explode all trees 17 ("nucleic acid" or "nucleic-acid amplification") near/5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*):ti,ab,kw (Word variations have been searched) 18 nat or naat:ti,ab,kw (Word variations have been searched) 19 ("reverse transcriptase" or "reverse transcription" or "real time") near/1 (PCR or "polymerase chain reaction"):ti,ab,kw (Word variations have been searched) 20 RT-PCR or RT-qPCR or PCR or qPCR:ti,ab,kw (Word variations have been searched) 21 (quantification or quantify or quantitative) near/1 (DNA or RNA):ti,ab,kw (Word variations have been searched) 22 (qualitative or descriptive) near/1 (DNA or RNA):ti,ab,kw (Word variations have been searched) 23 (rna or dna) near/5 (test or tests or testing or detect* or diagnos* or screen* or kit or kits or assay* or device*) .ti,ab,kw 24 #16 or #17 or #18 or #19 or #20 or #21 or #22 or #23 25 MeSH descriptor: [Sensitivity and Specificity] explode all trees 26 diagnos* accura* or sensitiv* or specific* or valid*:ti,ab,kw (Word variations have been searched) 27 "roc curve":ti,ab,kw (Word variations have been searched) 28 "positive predictive value":ti,ab,kw (Word variations have been searched) 29 "negative predictive value":ti,ab,kw (Word variations have been searched) 30 #25 or #26 or #27 or #28 or #29 31 #15 and #24 and #30 The search found 43 trials. Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS) (BIREME interface) LILACS was searched on 30 April 2015 ("hepatitis b" or "hep b" or "hbv" or "hbsag") and ("nucleic acid test$" or "nucleic acid detect$" or "nucleic acid diagnos$" or "nucleic acid screen$" or "nucleic acid assay$" or "nucleic-acid amplification" or nat or naat or "reverse transcriptase pcr" or "reverse transcription pcr" or "real time pcr" or "reverse transcriptase polymerase chain reaction" or "reverse transcription polymerase chain reaction" or "real time polymerase chain reaction" or rt-pcr or rt-qpcr or pcr or qpcr or "quantification dna" or "quantification rna" or "quantify dna" or "quantify rna" or "quantitative dna" or "quantitative rna" or "qualitative dna" or "qualitative rna" or "descriptive dna" or "descriptive rna" or "rna test$" or "dna test$" or "rna detect$" or "dna detect$" or "rna diagnos$" or "dna diagnos$" or "rna screen$" or "dna screen$" or "rna assay$" or "dna assay$") and ("diagnos$ accura$" or sensitiv$ or specific$ or valid$ or "roc curve" or "positive predictive value" or "negative predictive value") (20) WHO Global Index Medicus The database was searched on 30 April 2015 SUBJECT: (("Hepatitis, Viral, Human" OR "Hepatitis Viruses" OR "Hepatitis B virus" OR "Hepatitis Antibodies" OR "Hepadnaviridae Infections" OR "Hepatitis B Antibodies" OR "Hepatitis B Virus" OR "Hepadnaviridae" OR "Hepatitis B Surface Antigens") AND ("Nucleic Acid Amplification Techniques" OR "Enzyme-Linked Immunosorbent Assay" OR "Immunoassay" OR "Immunoenzyme Techniques") AND ("Sensitivity and Specificity")) (440)